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Gene regulatory factors of the sea urchin embryo. II. Two dissimilar proteins, P3A1 and P3A2, bind to the same target sites that are required for early territorial gene expression

Hoog, Christer and Calzone, Frank J. and Cutting, Ann E. and Britten, Roy J. and Davidson, Eric H. (1991) Gene regulatory factors of the sea urchin embryo. II. Two dissimilar proteins, P3A1 and P3A2, bind to the same target sites that are required for early territorial gene expression. Development, 112 (1). pp. 351-364. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:HOOdev91

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Abstract

Previous work demonstrated that a negative regulatory interaction mediated by factor(s) termed 'P3A' is required for correct territory-specific gene expression in the sea urchin embryo. A probe derived from a P3A target site in the skeletogenic SM50 gene of Strongylocentrotus purpuratus was used to isolate a cDNA clone coding for a factor that binds specifically to this site. This factor, called P3A1, contains two sequence elements that belong to the Zn finger class of DNA-binding motifs, and in these regions is most closely similar to the Drosophila hunchback factor. The P3A1 factor also binds to a similar target sequence in a second gene, CyIIIa, expressed in embryonic aboral ectoderm. Another sea urchin embryo protein factor, P3A2, has been isolated by affinity chromatography and cloned, as described in Calzone et al. Development 112, 335-350 (1991). P3A2 footprints the same target sites in the SM50 and CyIIIa genes as does P3A1, but lacks the Zn finger sequence motifs and in amino acid sequence is almost entirely dissimilar to P3A1. A deletion analysis of P3A2 delimited the DNA-binding region, revealing that five specific amino acids in the first P3A1 finger region and four in the second P3A1 finger region are also present in equivalent positions in P3A2. The P3A1 and P3A2 factors could function as regulatory antagonists, having evolved similar target specificities from dissimilar DNA-binding domains.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/112/1/351PublisherUNSPECIFIED
Additional Information:Copyright © 1991 by Company of Biologists. (Accepted 6 February 1991) We thank Mariann Brunkener for construction of the λgt11 library. This work was supported by an NIH grant HD-05753. Support for the initial phases were provided by a Developmental Biology grant from the Lucille P. Markey Charitable Trust C.H. was supported by a postdoctoral fellowship from EMBO.
Funders:
Funding AgencyGrant Number
National Institutes of HealthHD-05753
Lucille P. Markey Charitable TrustUNSPECIFIED
European Molecular Biology OrganizationUNSPECIFIED
Subject Keywords:DNA-binding protein, embryogenesis
Record Number:CaltechAUTHORS:HOOdev91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HOOdev91
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12612
Collection:CaltechAUTHORS
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Deposited On:15 Dec 2008 19:40
Last Modified:26 Dec 2012 10:36

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