Lee, S. W. and Yowanto, H. and Tai, Y. C. (1998) A micro cell lysis device. In: International Workshop on Micro Electro Mechanical Systems,11th (MEMS 98), Heidelberg, Germany, 25-29 January 1998. IEEE , Piscataway, NJ, pp. 443-447. ISBN 0-7803-4412-X http://resolver.caltech.edu/CaltechAUTHORS:LEEmems98
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A new micromachined cell lysis device is developed. It is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. It consists of muti-electrode pairs to apply electric fields to cells. We adopt the means of using electric field lysing because it can greatly simplify purification steps for preparation of biological samples, when compared to conventional chemical methods. Yeast, Chinese cabbage, radish cells and E. coli are tested with the device. The lysis of yeast, Chinese cabbage, radish cells is observed by a microscope. The experimental observation suggests E. coli are also lysed by the pulsed electric field. The range of electric field for the lysis is on the order of 1 kV/cm to 10 kV/cm. In addition, for practical reasons, we reduce the voltage required for lysing to less than 10 V by making the electrode gap on the order of microns.
|Item Type:||Book Section|
|Additional Information:||© Copyright 1998 IEEE. Reprinted with permission. Meeting Date: 01/25/1998 - 01/29/1998. This work is supported by the DARPA MICROFLUMES program under Naval Ocean Systems Center Contract N66001-96-C-83632. The authors would like to thank Xing Yang for help with processing.|
|Subject Keywords:||DNA; biological techniques; biomembranes; cellular biophysics; electrochemical analysis; micromechanical devices; Chinese cabbage; DNA identification; E. Coli; Yeast; biological sample; chemical methods; electric fields; electrode gap; intracellular materials; micro cell lysis device; micromachined cell lysis device; miniature bio-analysis; multi-electrode pairs; pulsed electric field; radish cells|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Kristin Buxton|
|Deposited On:||18 Dec 2008 04:34|
|Last Modified:||26 Dec 2012 10:37|
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