Tooker, Angela and Meng, Ellis and Erickson, Jon and Tai, Yu-Chong and Pine, Jerry (2004) Development of biocompatible parylene neurocages. In: International Conference of the IEEE Engineering in Medicine and Biology Society, 2th (IEMBS '04), San Francisco, CA, 1-5 September 2004. Vol.1. IEEE , Piscataway, NJ, pp. 2542-2545. ISBN 0-7803-8439-3 http://resolver.caltech.edu/CaltechAUTHORS:TOOembs04
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We present a refined method and design for building parylene neurocages for in vitro studies of live neural networks. Parylene neurocages are biocompatible and very robust, making them ideally suited for studying the synaptic connections between individual neurons to gain insight into learning and memory. The neurocage fabrication process is significantly less complex than earlier versions. Previous neurocage designs achieved limited neuronal outgrowth; however, the long-term cell survival rate was <25%. The incorporation of new materials and different anchoring techniques, in addition to some design modifications, as outlined here, have improved the long-term cell survival rate to >50%.
|Item Type:||Book Section|
|Additional Information:||© Copyright 2004 IEEE. Reprinted with permission. Publication Date: 1-5 Sept. 2004. This work was funded by the NIH under Award Number R01 NS044134. We would like to thank Mr. Trevor Roper, Mr. Qing He, and Ms. Angelique Johnson for assistance with fabrication and testing.|
|Subject Keywords:||neurocage; neuron; neuro-well; parylene; biotechnology; cellular biophysics; neural nets; neurophysiology; biocompatible parylene neurocages; learning; live neural network; long-term cell survival rate; memory; neurocage fabrication process; neuronal outgrowth; synaptic connection|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Kristin Buxton|
|Deposited On:||18 Dec 2008 05:36|
|Last Modified:||26 Dec 2012 10:37|
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