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Neural crest contributions to the lamprey head

McCauley, David W. and Bronner, Marianne E. (2003) Neural crest contributions to the lamprey head. Development, 130 (11). pp. 2317-2327. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:MCCdev03

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Abstract

The neural crest is a vertebrate-specific cell population that contributes to the facial skeleton and other derivatives. We have performed focal DiI injection into the cranial neural tube of the developing lamprey in order to follow the migratory pathways of discrete groups of cells from origin to destination and to compare neural crest migratory pathways in a basal vertebrate to those of gnathostomes. The results show that the general pathways of cranial neural crest migration are conserved throughout the vertebrates, with cells migrating in streams analogous to the mandibular and hyoid streams. Caudal branchial neural crest cells migrate ventrally as a sheet of cells from the hindbrain and super-pharyngeal region of the neural tube and form a cylinder surrounding a core of mesoderm in each pharyngeal arch, similar to that seen in zebrafish and axolotl. In addition to these similarities, we also uncovered important differences. Migration into the presumptive caudal branchial arches of the lamprey involves both rostral and caudal movements of neural crest cells that have not been described in gnathostomes, suggesting that barriers that constrain rostrocaudal movement of cranial neural crest cells may have arisen after the agnathan/gnathostome split. Accordingly, neural crest cells from a single axial level contributed to multiple arches and there was extensive mixing between populations. There was no apparent filling of neural crest derivatives in a ventral-to-dorsal order, as has been observed in higher vertebrates, nor did we find evidence of a neural crest contribution to cranial sensory ganglia. These results suggest that migratory constraints and additional neural crest derivatives arose later in gnathostome evolution.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.00451DOIUNSPECIFIED
http://dev.biologists.org/cgi/content/abstract/130/11/2317PublisherUNSPECIFIED
Additional Information:Copyright © 2003 The Company of Biologists Limited. Accepted 24 February 2003. We thank Roger Bergstedt and the staff at Hammond Bay Biological Station for facilities and assistance in lamprey collection and embryo culture; Robert Cerny for assistance with vibratome sectioning; Burcu Babaoglan for assistance with DiI labeling experiments; and Daniel Meulemans for many useful discussions. We also thank two anonymous reviewers for critical comments to improve this manuscript. This work was supported by NASA grant NAG 2-1585 to MBF and NIH training grant 5T32HD07257 to D.M.
Funders:
Funding AgencyGrant Number
NASANAG 2-1585
National Institutes of Health5T32HD07257
Subject Keywords:Cranial neural crest, Lamprey, DiI
Record Number:CaltechAUTHORS:MCCdev03
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MCCdev03
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12676
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:19 Dec 2008 05:37
Last Modified:26 Dec 2012 10:38

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