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Clonal variation in cell surface display of an H-2 protein lacking a cytoplasmic tail

Zuñiga, Martha C. and Hood, Leroy E. (1986) Clonal variation in cell surface display of an H-2 protein lacking a cytoplasmic tail. Journal of Cell Biology, 102 (1). pp. 1-10. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:ZUNjcb86

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Abstract

Truncated variants of the gene encoding H-2Ld, an integral membrane protein encoded by the major histocompatibility complex, were constructed by in vitro mutagenesis to elucidate the function of charged amino acids found on the cytoplasmic side of the transmembrane (TM) region. Analysis of cloned L cells transfected with these genes shows that the seven amino acids following the TM segment, four of which are basic, enhance the cell surface expression of H-2Ld protein but are not required for it. However, some clones do not express a tailless H-2Ld protein on the cell surface but express it intracellularly where it has a long half-life. Turnover measurements on cell surface H-2Ld proteins suggest that the basic residues following the TM segment are not a "stop transfer" sequence (Blobel, G., 1980, Proc. Natl. Acad. Sci. USA., 77:1496-1500) which anchors the H-2Ld protein in the membrane. Pulse-chase and endoglycosidase H sensitivity studies show that H-2Ld proteins lacking some or all of the basic residues and H-2Ld proteins which have a full-length cytoplasmic tail are processed with different kinetics. These results suggest an involvement of the membrane-proximal region of the cytoplasmic tail in the intracellular transport of H-2Ld. We further suggest that the L cell clones which do and do not express a tailless H-2Ld protein on the cell surface differ in the ability to transport a tailless integral membrane protein to the cell surface.


Item Type:Article
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http://jcb.rupress.org/cgi/content/abstract/102/1/1PublisherUNSPECIFIED
Additional Information:Copyright © 1986 by The Rockefeller University Press . RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 7 August 1985, and in revised form 12 September 1985. We thank Sarah Resnik, Gloria Park, and Debbie Maloney for technical assistance; Suzanne Horvath and Carol Graham for synthetic oligonucleotides; Bertha Jones and Connie Katz for preparation of the manuscript; Gunther Blobel, Paul Patterson, Scott Emr, and Andy Newman for advice and stimulating discussion during various stages of this work; and Steve Hunt, Joan Kobori, Rick Barth, Jeffrey Powell, Arlene Chiu, and Paul Patterson for critical reading of the manuscript. M.C. Zuniga was a fellow of the Cancer Research Institute. This work was supported by grant AI 19624 to L.E. Hood from the National Institutes of Health.
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Cancer Research InstituteUNSPECIFIED
National Institutes of HealthAI 19624
Record Number:CaltechAUTHORS:ZUNjcb86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ZUNjcb86
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:12699
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:20 Dec 2008 07:44
Last Modified:26 Dec 2012 10:38

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