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Scanning tunneling microscopy of DNA: Atom-resolved imaging, general observations and possible contrast mechanism

Youngquist, M. G. and Driscoll, R. J. and Coley, T. R. and Goddard, W. A. and Baldeschwieler, J. D. (1991) Scanning tunneling microscopy of DNA: Atom-resolved imaging, general observations and possible contrast mechanism. Journal of Vacuum Science and Technology B, 9 (2). pp. 1304-1308. ISSN 1071-1023. http://resolver.caltech.edu/CaltechAUTHORS:YOUjvstb91b

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Abstract

We have shown that it is possible to image DNA with atomic resolution using scanning tunneling microscopy (STM), [R. J. Driscoll, M. G. Youngquist, and J. D. Baldeschwieler, Nature 346, 294 (1990)]. Here we describe that data together with our general observations on STM of DNA in ultrahigh vacuum. We also suggest a possible contrast mechanism for DNA imaging by STM based on wave function orthogonality requirements between a molecule and its substrate. Topographic images are presented which resolve atomic features in addition to the double helical structure and nucleotide pairs of the DNA molecule. Comparisons of experimental STM profiles and modeled contours of the van der Waals surface of A-DNA show excellent correlation. Successive scans show that the imaging is nondestructive and reproducible. For this study, double-stranded DNA was deposited on highly oriented pyrolitic graphite without coating, shadowing, or chemical modification.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1116/1.585226DOIUNSPECIFIED
Additional Information:© 1991 American Vacuum Society. Received 24 July 1990; accepted 18 September 1990. We wish to thank Charles Spence and Joseph Meier of Dr. Leroy Hood's group in the Caltech Biology division for persuading us to undertake this project and for preparing the DNA solution, and Jonathan Hurley and Manish Sud of Biodesign Inc. for molecular modeling software. MGY is a Dept. of Education fellow. This work has been supported by grants from NIH, ONR, Shell Companies Foundation, and the NSF.
Funders:
Funding AgencyGrant Number
Department of EducationUNSPECIFIED
National Institutes of HealthUNSPECIFIED
Office of Naval ResearchUNSPECIFIED
Shell Companies FoundationUNSPECIFIED
National Science FoundationUNSPECIFIED
Subject Keywords:DNA, GRAPHITE, SUBSTRATES, IMAGES, HIGH–RESOLUTION METHODS, SCANNING ELECTRON MICROSCOPY, ULTRAHIGH VACUUM, WAVE FUNCTIONS, MATHEMATICAL MODELS
Record Number:CaltechAUTHORS:YOUjvstb91b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:YOUjvstb91b
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12719
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:21 Dec 2008 03:33
Last Modified:26 Dec 2012 10:39

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