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The C. elegans LIM homeobox gene lin-11 specifies multiple cell fates during vulval development

Gupta, Bhagwati P. and Wang, Minqin and Sternberg, Paul W. (2003) The C. elegans LIM homeobox gene lin-11 specifies multiple cell fates during vulval development. Development, 130 (12). pp. 2589-2601. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:GUPdev03

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Abstract

LIM homeobox family members regulate a variety of cell fate choices during animal development. In C. elegans, mutations in the LIM homeobox gene lin-11 have previously been shown to alter the cell division pattern of a subset of the 2° lineage vulval cells. We demonstrate multiple functions of lin-11 during vulval development. We examined the fate of vulval cells in lin-11 mutant animals using five cellular markers and found that lin-11 is necessary for the patterning of both 1° and 2° lineage cells. In the absence of lin-11 function, vulval cells fail to acquire correct identity and inappropriately fuse with each other. The expression pattern of lin-11 reveals dynamic changes during development. Using a temporally controlled overexpression system, we show that lin-11 is initially required in vulval cells for establishing the correct invagination pattern. This process involves asymmetric expression of lin-11 in the 2° lineage cells. Using a conditional RNAi approach, we show that lin-11 regulates vulval morphogenesis. Finally, we show that LDB-1, a NLI/Ldb1/CLIM2 family member, interacts physically with LIN-11, and is necessary for vulval morphogenesis. Together, these findings demonstrate that temporal regulation of lin-11 is crucial for the wild-type vulval patterning.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/10.1242/dev.00500DOIUNSPECIFIED
http://dev.biologists.org/cgi/content/abstract/130/12/2589PublisherUNSPECIFIED
Additional Information:Copyright © 2003 The Company of Biologists Limited. Accepted 17 March 2003. We thank S. Cameron, D. Sherwood and T. Inoue for integrated strains. Robert Oania provided assistance in two-hybrid experiments. Some of the strains were obtained from Caenorhabditis Genetics Center. We also thank D. Sherwood, T. Inoue and anonymous reviewers for comments on the manuscript. This work was supported by funds from the HHMI and USPHS grant HD23690 to P.W.S. B.P.G. was supported by a long-term fellowship from HFSP and as an associate of the HHMI. M.W. was an AMGEN fellow. P.W.S. is an investigator of the HHMI.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical InstituteUNSPECIFIED
Public Health ServiceHD23690
Human Frontier Science ProgramUNSPECIFIED
Amgen Corp.UNSPECIFIED
Subject Keywords:C. elegans, lin-11, ldb-1, LIM homeodomain, Vulva, Differentiation
Record Number:CaltechAUTHORS:GUPdev03
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GUPdev03
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12787
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:23 Dec 2008 22:42
Last Modified:26 Dec 2012 10:40

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