Gordon, Sean P. and Heisler, Marcus G. and Reddy, G. Venugopala and Ohno, Carolyn and Das, Pradeep and Meyerowitz, Elliot M. (2007) Pattern formation during de novo assembly of the Arabidopsis shoot meristem. Development, 134 (19). pp. 3539-3548. ISSN 0950-1991 http://resolver.caltech.edu/CaltechAUTHORS:GORdev07
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Most multicellular organisms have a capacity to regenerate tissue after wounding. Few, however, have the ability to regenerate an entire new body from adult tissue. Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. We characterize early patterning during de novo development of the Arabidopsis shoot meristem using fluorescent reporters of known gene and protein activities required for shoot meristem development and maintenance. We find that a small number of progenitor cells initiate development of new shoot meristems through stereotypical stages of reporter expression and activity of CUP-SHAPED COTYLEDON 2 (CUC2), WUSCHEL (WUS), PIN-FORMED 1 (PIN1), SHOOT-MERISTEMLESS (STM), FILAMENTOUS FLOWER (FIL, also known as AFO), REVOLUTA (REV), ARABIDOPSIS THALIANA MERISTEM L1 LAYER (ATML1) and CLAVATA 3 (CLV3). Furthermore, we demonstrate a functional requirement for WUS activity during de novo shoot meristem initiation. We propose that de novo shoot meristem induction is an easily accessible system for the study of patterning and self-organization in the well-studied model organism Arabidopsis.
|Additional Information:||© The Company of Biologists Ltd 2007. Accepted 21 July 2007. First published online September 7, 2007 doi: 10.1242/10.1242/dev.010298 We thank Joe Keiber for the ARR5::GFP seeds. We thank Kaoru Sugimoto for assistance with the supplemental RT-PCR data, Annick Dubois and Arnavaz Garda for technical advice, and Elizabeth Haswell for critical comments on the manuscript. This work was funded by National Science Foundation grant IOS-0211670 to E.M.M. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/134/19/3539/DC1|
|Subject Keywords:||Auxin, Callus, Cytokinin, Regeneration, Self-organization, Shoot meristem, Arabidopsis thaliana|
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|Deposited By:||Archive Administrator|
|Deposited On:||11 Jan 2009 05:40|
|Last Modified:||26 Dec 2012 10:42|
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