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An intragenic revertant of a poliovirus 2C mutant has an uncoating defect

Li, Jing-Po and Baltimore, David (1990) An intragenic revertant of a poliovirus 2C mutant has an uncoating defect. Journal of Virology, 64 (3). pp. 1102-1107. ISSN 0022-538X. PMCID PMC249223. http://resolver.caltech.edu/CaltechAUTHORS:LIJjvir90

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Abstract

A revertant was isolated from a temperature-sensitive poliovirus 2C mutant, 2C-31, which is defective in viral RNA synthesis. This revertant, called 2C-31R1, grew well at 39 degrees C and was not defective in RNA synthesis. However, in contrast to its parental mutant, 2C-31R1 was cold sensitive and could hardly grow at all at 32 degrees C. Analysis of a single-cycle growth revealed that 2C-31R1 was defective in virion uncoating at 32 degrees C, and a substantial amount (more than 30%) of input viruses could be recovered as infectious particles from an infected cell lysate up to 6 h postinfection. The uncoating defect and the inability to grow at cold temperatures could be overcome by a brief incubation at the permissive temperature (39 degrees C) before the infection was continued at 32 degrees C. cDNA cloning and mix-and-match recombination experiments indicated that the defect in uncoating was the result of two secondary point mutations, seven nucleotides apart, in the 2C-coding sequence downstream of the inserted linker which is the original mutation in the parental 2C-31 genome. Another revertant, 2C-31R3, isolated from the same 2C-31 stock, was not defective in uncoating and appeared to be a secondary revertant that contained an intragenic suppressor for the uncoating defect. The uncoating defect of 2C-31R1 could be complemented by type 2 poliovirus. These results suggested that protein 2C, in addition to its role in viral RNA synthesis, has a function in determining virion structure.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jvi.asm.org/cgi/content/abstract/64/3/1102PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC249223/PubMed CentralArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1990 American Society for Microbiology. Received 18 July 1989/Accepted 16 November 1989. This work was supported by Public Health Service grant AI 22346 from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
NIHAI 22346
PubMed Central ID:PMC249223
Record Number:CaltechAUTHORS:LIJjvir90
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LIJjvir90
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13029
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:15 Jan 2009 23:39
Last Modified:04 Apr 2017 23:02

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