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An intragenic revertant of a poliovirus 2C mutant has an uncoating defect

Li, Jing-Po and Baltimore, David (1990) An intragenic revertant of a poliovirus 2C mutant has an uncoating defect. Journal of Virology, 64 (3). pp. 1102-1107. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:LIJjvir90

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Abstract

A revertant was isolated from a temperature-sensitive poliovirus 2C mutant, 2C-31, which is defective in viral RNA synthesis. This revertant, called 2C-31R1, grew well at 39 degrees C and was not defective in RNA synthesis. However, in contrast to its parental mutant, 2C-31R1 was cold sensitive and could hardly grow at all at 32 degrees C. Analysis of a single-cycle growth revealed that 2C-31R1 was defective in virion uncoating at 32 degrees C, and a substantial amount (more than 30%) of input viruses could be recovered as infectious particles from an infected cell lysate up to 6 h postinfection. The uncoating defect and the inability to grow at cold temperatures could be overcome by a brief incubation at the permissive temperature (39 degrees C) before the infection was continued at 32 degrees C. cDNA cloning and mix-and-match recombination experiments indicated that the defect in uncoating was the result of two secondary point mutations, seven nucleotides apart, in the 2C-coding sequence downstream of the inserted linker which is the original mutation in the parental 2C-31 genome. Another revertant, 2C-31R3, isolated from the same 2C-31 stock, was not defective in uncoating and appeared to be a secondary revertant that contained an intragenic suppressor for the uncoating defect. The uncoating defect of 2C-31R1 could be complemented by type 2 poliovirus. These results suggested that protein 2C, in addition to its role in viral RNA synthesis, has a function in determining virion structure.


Item Type:Article
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http://jvi.asm.org/cgi/content/abstract/64/3/1102PublisherUNSPECIFIED
Additional Information:Copyright © 1990 by the American Society for Microbiology. Received 18 July 1989/Accepted 16 November 1989. This work was supported by Public Health Service grant AI 22346 from the National Institutes of Health.
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National Institutes of HealthAI 22346
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Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LIJjvir90
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Deposited On:15 Jan 2009 23:39
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