A Caltech Library Service

An intragenic revertant of a poliovirus 2C mutant has an uncoating defect

Li, Jing-Po and Baltimore, David (1990) An intragenic revertant of a poliovirus 2C mutant has an uncoating defect. Journal of Virology, 64 (3). pp. 1102-1107. ISSN 0022-538X.

PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


A revertant was isolated from a temperature-sensitive poliovirus 2C mutant, 2C-31, which is defective in viral RNA synthesis. This revertant, called 2C-31R1, grew well at 39 degrees C and was not defective in RNA synthesis. However, in contrast to its parental mutant, 2C-31R1 was cold sensitive and could hardly grow at all at 32 degrees C. Analysis of a single-cycle growth revealed that 2C-31R1 was defective in virion uncoating at 32 degrees C, and a substantial amount (more than 30%) of input viruses could be recovered as infectious particles from an infected cell lysate up to 6 h postinfection. The uncoating defect and the inability to grow at cold temperatures could be overcome by a brief incubation at the permissive temperature (39 degrees C) before the infection was continued at 32 degrees C. cDNA cloning and mix-and-match recombination experiments indicated that the defect in uncoating was the result of two secondary point mutations, seven nucleotides apart, in the 2C-coding sequence downstream of the inserted linker which is the original mutation in the parental 2C-31 genome. Another revertant, 2C-31R3, isolated from the same 2C-31 stock, was not defective in uncoating and appeared to be a secondary revertant that contained an intragenic suppressor for the uncoating defect. The uncoating defect of 2C-31R1 could be complemented by type 2 poliovirus. These results suggested that protein 2C, in addition to its role in viral RNA synthesis, has a function in determining virion structure.

Item Type:Article
Related URLs:
URLURL TypeDescription
Additional Information:Copyright © 1990 by the American Society for Microbiology. Received 18 July 1989/Accepted 16 November 1989. This work was supported by Public Health Service grant AI 22346 from the National Institutes of Health.
Funding AgencyGrant Number
National Institutes of HealthAI 22346
Record Number:CaltechAUTHORS:LIJjvir90
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13029
Deposited By: Archive Administrator
Deposited On:15 Jan 2009 23:39
Last Modified:26 Dec 2012 10:43

Repository Staff Only: item control page