Chiu, Chi-Sung and Ross, Linda S. and Cohen, Bruce N. and Lester, Henry A. and Gill, Sarjeet S. (2000) The transporter-like protein inebriated mediates hyperosmotic stimuli through intracellular signaling. Journal of Experimental Biology, 203 (23). pp. 3531-3546. ISSN 0022-0949 http://resolver.caltech.edu/CaltechAUTHORS:CHIjeb00
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We cloned the inebriated homologue MasIne from Manduca sexta and expressed it in Xenopus laevis oocytes. MasIne is homologous to neurotransmitter transporters but no transport was observed with a number of putative substrates. Oocytes expressing MasIne respond to hyperosmotic stimulation by releasing intracellular Ca(2+), as revealed by activation of the endogenous Ca(2+)-activated Cl(-) current. This Ca(2+) release requires the N-terminal 108 amino acid residues of MasIne and occurs via the inositol trisphosphate pathway. Fusion of the N terminus to the rat gamma-aminobutyric acid transporter (rGAT1) also renders rGAT1 responsive to hyperosmotic stimulation. Immunohistochemical analyses show that MasIne and Drosophila Ine have similar tissue distribution patterns, suggesting functional identity. Inebriated is expressed in tissues and cells actively involved in K(+) transport, which suggests that it may have a role in ion transport, particularly of K(+). We propose that stimulation of MasIne releases intracellular Ca(2+) in native tissues, activating Ca(2+)-dependent K(+) channels, and leading to K(+) transport.
|Additional Information:||© The Company of Biologists Limited 2000. Accepted 7 September; published on WWW 2 November 2000. We thank M. E. Adams, C. Y. Lytle, M. J. Schuler, D. Zitnan, A. K. Pullikuth, C. Labarca, M. Filippova, A. Kondo and L. McCloud for helpful discussion and technical support. This research was supported by grants from the NIH (AI34524 and AI48049 to S.S.G. and DA-09121 to H.A.L.).|
|Subject Keywords:||Manduca sexta; inebriated; Ca2+-activated Cl- current; inositol trisphosphate; permeable phospholipase C|
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|Deposited By:||Tony Diaz|
|Deposited On:||11 Feb 2009 03:53|
|Last Modified:||26 Dec 2012 10:47|
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