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The transporter-like protein inebriated mediates hyperosmotic stimuli through intracellular signaling

Chiu, Chi-Sung and Ross, Linda S. and Cohen, Bruce N. and Lester, Henry A. and Gill, Sarjeet S. (2000) The transporter-like protein inebriated mediates hyperosmotic stimuli through intracellular signaling. Journal of Experimental Biology, 203 (23). pp. 3531-3546. ISSN 0022-0949. http://resolver.caltech.edu/CaltechAUTHORS:CHIjeb00

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Abstract

We cloned the inebriated homologue MasIne from Manduca sexta and expressed it in Xenopus laevis oocytes. MasIne is homologous to neurotransmitter transporters but no transport was observed with a number of putative substrates. Oocytes expressing MasIne respond to hyperosmotic stimulation by releasing intracellular Ca(2+), as revealed by activation of the endogenous Ca(2+)-activated Cl(-) current. This Ca(2+) release requires the N-terminal 108 amino acid residues of MasIne and occurs via the inositol trisphosphate pathway. Fusion of the N terminus to the rat gamma-aminobutyric acid transporter (rGAT1) also renders rGAT1 responsive to hyperosmotic stimulation. Immunohistochemical analyses show that MasIne and Drosophila Ine have similar tissue distribution patterns, suggesting functional identity. Inebriated is expressed in tissues and cells actively involved in K(+) transport, which suggests that it may have a role in ion transport, particularly of K(+). We propose that stimulation of MasIne releases intracellular Ca(2+) in native tissues, activating Ca(2+)-dependent K(+) channels, and leading to K(+) transport.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jeb.biologists.org/cgi/content/abstract/203/23/3531PublisherUNSPECIFIED
Additional Information:© The Company of Biologists Limited 2000. Accepted 7 September; published on WWW 2 November 2000. We thank M. E. Adams, C. Y. Lytle, M. J. Schuler, D. Zitnan, A. K. Pullikuth, C. Labarca, M. Filippova, A. Kondo and L. McCloud for helpful discussion and technical support. This research was supported by grants from the NIH (AI34524 and AI48049 to S.S.G. and DA-09121 to H.A.L.).
Funders:
Funding AgencyGrant Number
National Institutes of HealthAI4524
National Institutes of HealthAI48049
National Institutes of HealthDA-09121
Subject Keywords:Manduca sexta; inebriated; Ca2+-activated Cl- current; inositol trisphosphate; permeable phospholipase C
Record Number:CaltechAUTHORS:CHIjeb00
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHIjeb00
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13265
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Feb 2009 03:53
Last Modified:26 Dec 2012 10:47

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