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Non-autonomy of AGAMOUS function in flower development: use of a Cre/loxP method for mosaic analysis in Arabidopsis

Sieburth, Leslie E. and Drews, Gary N. and Meyerowitz, Elliot M. (1998) Non-autonomy of AGAMOUS function in flower development: use of a Cre/loxP method for mosaic analysis in Arabidopsis. Development, 125 (21). pp. 4303-4312. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:SIEdev98

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Abstract

Angiosperms use a multi-layered meristem (typically L1, L2 and L3) to produce primordia that then develop into plant organs, A number of experiments show that communication between the cell layers is important for normal development. We examined whether the function of the flower developmental control gene AGAMOUS involves communication across these layers. We developed a mosaic strategy using the Cre/loxP site-specific recombinase system, and identified the sector structure for mosaics that produced mutant flowers. The major conclusions were that (1) AGAMOUS must be active in the L2 for staminoid and carpelloid tissues, (2) that AGAMOUS must be active in the L2 and the L3 for floral meristem determinacy, and (3) that epidermal cell identity can be communicated by the L2 to the L1 layer.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/125/21/4303PublisherUNSPECIFIED
Additional Information:© The Company of Biologists Limited 1998. Accepted 10 August; published on WWW 30 September 1998. We gratefully acknowledge members of the Meyerowitz lab for useful discussions of this work, and Dr Robert Goldberg, in whose lab the plant transformations were performed. This work was supported by a grant from the National Institutes of Health (GM45697 to E. M. M.), a grant from the National Sciences and Engineering Research Council of Canada (OGP0170655 to L. E. S), by a Damon Runyon-Walter Winchell Cancer Research Fellowship (DRG1081 to L. E. S.), and by an NIH Postdoctoral Fellowship (GM13100-03 to G. N. D.). We also thank Jennifer Fletcher, Doris Wagner, Jose-Luis Reichmann, Toshiro Ito, Kevin Roberg, and Prakash Kumar for critical reading of the manuscript.
Funders:
Funding AgencyGrant Number
NIHGM45697
National Sciences and Engineering Research Council of CanadaOGP0170655
Damon Runyon-Walter Winchell Cancer Research FellowshipDRG1081
NIH Postdoctoral FellowshipGM13100-03
Subject Keywords:flower development; AGAMOUS; genetic mosaics; Arabidopsis; meristem
Record Number:CaltechAUTHORS:SIEdev98
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SIEdev98
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13377
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Apr 2009 17:22
Last Modified:26 Dec 2012 10:49

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