Klein, William H. and Murphy, William and Attardi, Giuseppe and Britten, Roy J. and Davidson, Eric H. (1974) Distribution of repetitive and nonrepetitive sequence transcripts in HeLa mRNA. Proceedings of the National Academy of Sciences of the United States of America, 71 (5). pp. 1785-1789. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:KLEpnas74
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Polyadenylated messenger RNA extracted from HeLa cells was hybridized with a mass excess of HeLa DNA. The kinetics of the hybridization reaction demonstrated that most of the messenger RNA is transcribed from nonrepetitive DNA. The amount of messenger RNA hybridized to DNA was measured both with and without prior RNase treatment. Comparison of the results indicates that within the limits of detection, HeLa messenger RNA does not contain repetitive sequence elements covalently linked to nonrepetitive sequence transcripts. However, a small fraction of the HeLa messenger RNA preparation is transcribed entirely from repetitive DNA sequences. This fraction represents about 6% of the total polyadenylated messenger RNA preparation.
|Additional Information:||Copyright © 1974 by the National Academy of Sciences. Contributed by Roy J. Britten, February 1, 1974. The help of Mrs. Arger Drew is gratefully acknowledged. This research was supported by an NSF Grant GB-33441 and by National Institutes of Health Grants HD-05753 and GM-11726.|
|Subject Keywords:||polyadenylated mRNA, hydroxyapatite, RNA-DNA hybridization|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||13 Jan 2006|
|Last Modified:||26 Dec 2012 08:44|
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