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Distribution of repetitive and nonrepetitive sequence transcripts in HeLa mRNA

Klein, William H. and Murphy, William and Attardi, Giuseppe and Britten, Roy J. and Davidson, Eric H. (1974) Distribution of repetitive and nonrepetitive sequence transcripts in HeLa mRNA. Proceedings of the National Academy of Sciences of the United States of America, 71 (5). pp. 1785-1789. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KLEpnas74

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Abstract

Polyadenylated messenger RNA extracted from HeLa cells was hybridized with a mass excess of HeLa DNA. The kinetics of the hybridization reaction demonstrated that most of the messenger RNA is transcribed from nonrepetitive DNA. The amount of messenger RNA hybridized to DNA was measured both with and without prior RNase treatment. Comparison of the results indicates that within the limits of detection, HeLa messenger RNA does not contain repetitive sequence elements covalently linked to nonrepetitive sequence transcripts. However, a small fraction of the HeLa messenger RNA preparation is transcribed entirely from repetitive DNA sequences. This fraction represents about 6% of the total polyadenylated messenger RNA preparation.


Item Type:Article
Additional Information:Copyright © 1974 by the National Academy of Sciences. Contributed by Roy J. Britten, February 1, 1974. The help of Mrs. Arger Drew is gratefully acknowledged. This research was supported by an NSF Grant GB-33441 and by National Institutes of Health Grants HD-05753 and GM-11726.
Subject Keywords:polyadenylated mRNA, hydroxyapatite, RNA-DNA hybridization
Record Number:CaltechAUTHORS:KLEpnas74
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KLEpnas74
Alternative URL:http://www.pnas.org/cgi/content/abstract/71/5/1785
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1377
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Jan 2006
Last Modified:26 Dec 2012 08:44

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