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Instability of GGL domain-containing RGS proteins in mice lacking the G protein β-subunit Gβ5

Chen, Ching-Kang and Eversole-Cire, Pamela and Zhang, Haikun and Mancino, Valeria and Chen, Yu-Jiun and He, Wei and Wensel, Theodore G. and Simon, Melvin I. (2003) Instability of GGL domain-containing RGS proteins in mice lacking the G protein β-subunit Gβ5. Proceedings of the National Academy of Sciences of the United States of America, 100 (11). pp. 6604-6609. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:CHEpnas03

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Abstract

RGS (regulator of G protein signaling) proteins containing the G protein gamma-like (GGL) domain (RGS6, RGS7, RGS9, and RGS11) interact with the fifth member of the G protein beta-subunit family, Gbeta5. This interaction is necessary for the stability of both the RGS protein and for Gbeta5. Consistent with this notion, we have found that elevation of RGS9-1 mRNA levels by transgene expression does not increase RGS9-1 protein level in the retina, suggesting that Gbeta5 levels may be limiting. To examine further the interactions of Gbeta5 and the GGL domain-containing RGS proteins, we inactivated the Gbeta5 gene. We found that the levels of GGL domain-containing RGS proteins in retinas and in striatum are eliminated or reduced drastically, whereas the levels of G,2 and RGS4 proteins remain normal in the absence of Gbeta5. The homozygous Gbeta5 knockout (Gbeta5(-/-)) mice derived from heterozygous knockout mating are runty and exhibit a high preweaning mortality rate. We concluded that complex formation between GGL domain-containing RGS proteins and the Gbeta5 protein is necessary to maintain their mutual stability in vivo. Furthermore, in the absence of Gbeta5 and all four RGS proteins that form protein complexes with Gbeta5, the animals that survive into adulthood are viable and have no gross defects in brain or retinal morphology.


Item Type:Article
Additional Information:Copyright © 2003 by the National Academy of Sciences. Contributed by Melvin I. Simon, March 28, 2003. Published online before print May 8, 2003, 10.1073/pnas.0631825100. We thank Alan John Watson, Marie Burns, and Wolfgang Baehr for helpful discussion and Yi-Hui Hu and the Caltech Transgenic Core Facility for technical assistance. C.-K.C. is a recipient of the Research to Prevent Blindness Career Development Award. This work was supported by National Institutes of Health Grants EY013811 (C.-K.C.) and AG12288 (M.I.S.).
Subject Keywords:GTPASE-ACCELERATING PROTEIN, CGMP-PHOSPHODIESTERASE, ACTIVATING PROTEINS, ALPHA-SUBUNITS, SIGNALING RGS, RD MOUSE, EXPRESSION, GENE, ASSOCIATION, REGULATORS
Record Number:CaltechAUTHORS:CHEpnas03
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHEpnas03
Alternative URL:http://dx.doi.org/10.1073/pnas.0631825100
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1414
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Jan 2006
Last Modified:14 Nov 2014 19:18

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