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Published March 19, 2009 | Supplemental Material + Accepted Version
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Iterative in situ click chemistry creates antibody-like protein-capture agents

Agnew, Heather D.
Rohde, Rosemary D.
Millward, Steven W.
Nag, Arundhati
Yeo, Woon-Seok
Hein, Jason E.
Pitram, Suresh M.
Tariq, Abdul Ahad
Burns, Vanessa M.
Krom, Russell J.
Fokin, Valery V.
Sharpless, K. Barry ORCID icon
Heath, James R. ORCID icon

Abstract

Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper (I)-catalyzed cycloaddition.

Additional Information

© 2009 Wiley VCH Verlag GmbH & Co. KGaA, Weinheim. Received: January 26, 2009. Published online: March 19, 2009. This research was supported by the National Cancer Institute, grant No. 5U54 CA119347 (J.R.H.), the W. M. Keck Foundation (K.B.S.), and a subcontract from the MITRE Corporation (J.R.H.). Postdoctoral and graduate fellowships were provided by the NSF and PEO (H.D.A.), the Gates Foundation (R.D.R.), the NSERC (J.E.H.), and the NCI (S.W.M.). We also acknowledge H. C. Kolb, S. S. Lee, J. Cha, J. Lim, S. Tan, S. Y. Yeo, A. Srivastava, M. Barrientos, E. Johnson Chavarria, A. Stuparu, J. Vielmetter, and C. S. Parker for useful discussions and assistance.

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Supplemental Material - sm001.pdf

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October 18, 2023