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Visualization of RNA tertiary struture by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47

Han, Hogyu and Dervan, Peter B. (1994) Visualization of RNA tertiary struture by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA·Fe(II) at position 47. Proceedings of the National Academy of Sciences of the United States of America, 91 (11). pp. 4955-4959. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:HANpnas94

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Abstract

To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA.Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)(2)(SO4)(2) and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.


Item Type:Article
Additional Information:Copyright © 1994 by the National Academy of Sciences. Contributed by Peter B. Dervan, February 24, 1993. We are grateful to the National Institutes of Health for grant support. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:AFFINITY CLEAVING; YEAST TRNA(PHE); CLEAVAGE OF RNA; PHENYLALANINE TRANSFER-RNA; SEQUENCE-SPECIFIC CLEAVAGE; YEAST TRANSFER-RNAPHE; TRIPLE-HELIX FORMATION; BINDING-SITES; DNA; MOLECULES; RECOGNITION; RESOLUTION; SYNTHETASE
Record Number:CaltechAUTHORS:HANpnas94
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HANpnas94
Alternative URL:http://www.pnas.org/cgi/content/abstract/91/11/4955
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1500
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Jan 2006
Last Modified:14 Nov 2014 19:19

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