Rich, Rebecca L. and Papalia, Giuseppe A. and Flynn, Peter J. and Furneisen, Jamie and Quinn, John and Klein, Joshua S. and Katsamba, Phini S. and Waddell, M. Brent and Scott, Michael and Thompson, Joshua and Berlier, Judie and Corry, Schuyler and Baltzinger, Mireille and Zeder-Lutz, Gabrielle and Schoenemann, Andreas and Clabbers, Anca and Wieckowski, Sebastien and Murphy, Mary M. and Page, Phillip and Ryan, Thomas E. and Duffner, Jay and Ganguly, Tanmoy and Corbin, John and Gautam, Satyen and Anderluh, Gregor and Bavdek, Andrej and Reichmann, Dana and Yadav, Satya P. and Hommema, Eric and Pol, Ewa and Drake, Andrew and Klakamp, Scott and Chapman, Trevor and Kernaghan, Dawn and Miller, Ken and Schuman, Jason and Lindquist, Kevin and Herlihy, Kara and Murphy, Michael B. and Bohnsack, Richard and Andrien, Bruce and Brandani, Pietro and Terwey, Danny and Millican, Rohn and Darling, Ryan J. and Wangae, Liann and Carter, Quincy and Dotzlaf, Joe and Lopez-Sagaseta, Jacinto and Campbell, Islay and Torreri, Paola and Hoos, Sylviane and England, Patrick and Liu, Yang and Abdiche, Yasmina and Malashock, Daniel and Pinkerton, Alanna and Wong, Melanie and Lafer, Eileen and Hinck, Cynthia and Thompson, Kevin and Di Primo, Carmelo and Joyce, Alison and Brooks, Jonathan and Torta, Federico and Bagge Hagel, Anne Birgitte and Krarup, Janus and Pass, Jesper and Ferreira, Monica and Shikov, Sergei and Mikolajczyk, Malgorzata and Abe, Yuki and Barbato, Gaetano and Giannetti, Anthony M. and Krishnamoorthy, Ganeshram and Beusink, Bianca and Satpaev, Daulet and Tsang, Tiffany and Fang, Eric and Partridge, James and Brohawn, Stephen and Horn, James and Pritsch, Otto and Obal, Gonzalo and Nilapwar, Sanjay and Busby, Ben and Gutierrez-Sanchez, Gerardo and Das Gupta, Ruchira and Canepa, Sylvie and Witte, Krista and Nikolovska-Coleska, Zaneta and Cho, Yun Hee and D’Agata, Roberta and Schlick, Kristian and Calvert, Rosy and Munoz, Eva M. and Hernaiz, Maria Jose and Bravman, Tsafir and Dines, Monica and Yang, Min-Hsiang and Puskas, Agnes and Boni, Erica and Li, Jiejin and Wear, Martin and Grinberg, Asya and Baardsnes, Jason and Dolezal, Olan and Gainey, Melicia and Anderson, Henrik and Peng, Jinlin and Lewis, Mark and Spies, Peter and Trinh, Quyhn and Bibikov, Sergei and Raymond, Jill and Yousef, Mohammed and Chandrasekaran, Vidya and Feng, Yuguo and Emerick, Anne and Mundodo, Suparna and Guimaraes, Rejane and McGirr, Katy and Li, Yue-Ji and Hughes, Heather and Mantz, Hubert and Skrabana, Rostislav and Witmer, Mark and Ballard, Joshua and Martin, Loic and Skladal, Petr and Korza, George and Laird-Offringa, Ite and Lee, Charlene S. and Khadir, Abdelkrim and Podlaski, Frank and Neuner, Phillippe and Rothacker, Julie and Rafique, Ashique and Dankbar, Nico and Kainz, Peter and Gedig, Erk and Vuyisich, Momchilo and Boozer, Christina and Ly, Nguyen and Toews, Mark and Uren, Aykut and Kalyuzhniy, Oleksandr and Lewis, Kenneth and Chomey, Eugene and Pak, Brian J. and Myszka, David G. (2009) A global benchmark study using affinity-based biosensors. Analytical Biochemistry, 386 (2). pp. 194-216. ISSN 0003-2697 http://resolver.caltech.edu/CaltechAUTHORS:20090901-094826364
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To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
|Additional Information:||© 2009 Elsevier B.V. Received 3 October 2008. Available online 27 November 2008. We thank KaloBios Pharmaceuticals for providing the purified Fab and GST–Ag, Biacore/GE Healthcare for providing sensor chips to help develop this model system, and Bio-Rad Laboratories for shipping all of the sample sets worldwide.|
|Subject Keywords:||Biacore; Kinetics; Optical biosensor; Surface plasmon resonance|
|Official Citation:||Rebecca L. Rich, Giuseppe A. Papalia, Peter J. Flynn, Jamie Furneisen, John Quinn, Joshua S. Klein, Phini S. Katsamba, M. Brent Waddell, Michael Scott, Joshua Thompson, Judie Berlier, Schuyler Corry, Mireille Baltzinger, Gabrielle Zeder-Lutz, Andreas Schoenemann, Anca Clabbers, Sebastien Wieckowski, Mary M. Murphy, Phillip Page, Thomas E. Ryan, Jay Duffner, Tanmoy Ganguly, John Corbin, Satyen Gautam, Gregor Anderluh, Andrej Bavdek, Dana Reichmann, Satya P. Yadav, Eric Hommema, Ewa Pol, Andrew Drake, Scott Klakamp, Trevor Chapman, Dawn Kernaghan, Ken Miller, Jason Schuman, Kevin Lindquist, Kara Herlihy, Michael B. Murphy, Richard Bohnsack, Bruce Andrien, Pietro Brandani, Danny Terwey, Rohn Millican, Ryan J. Darling, Liann Wang, Quincy Carter, Joe Dotzlaf, Jacinto Lopez-Sagaseta, Islay Campbell, Paola Torreri, Sylviane Hoos, Patrick England, Yang Liu, Yasmina Abdiche, Daniel Malashock, Alanna Pinkerton, Melanie Wong, Eileen Lafer, Cynthia Hinck, Kevin Thompson, Carmelo Di Primo, Alison Joyce, Jonathan Brooks, Federico Torta, Anne Birgitte Bagge Hagel, Janus Krarup, Jesper Pass, Monica Ferreira, Sergei Shikov, Malgorzata Mikolajczyk, Yuki Abe, Gaetano Barbato, Anthony M. Giannetti, Ganeshram Krishnamoorthy, Bianca Beusink, Daulet Satpaev, Tiffany Tsang, Eric Fang, James Partridge, Stephen Brohawn, James Horn, Otto Pritsch, Gonzalo Obal, Sanjay Nilapwar, Ben Busby, Gerardo Gutierrez-Sanchez, Ruchira Das Gupta, Sylvie Canepa, Krista Witte, Zaneta Nikolovska-Coleska, Yun Hee Cho, Roberta D'Agata, Kristian Schlick, Rosy Calvert, Eva M. Munoz, Maria Jose Hernaiz, Tsafir Bravman, Monica Dines, Min-Hsiang Yang, Agnes Puskas, Erica Boni, Jiejin Li, Martin Wear, Asya Grinberg, Jason Baardsnes, Olan Dolezal, Melicia Gainey, Henrik Anderson, Jinlin Peng, Mark Lewis, Peter Spies, Quyhn Trinh, Sergei Bibikov, Jill Raymond, Mohammed Yousef, Vidya Chandrasekaran, Yuguo Feng, Anne Emerick, Suparna Mundodo, Rejane Guimaraes, Katy McGirr, Yue-Ji Li, Heather Hughes, Hubert Mantz, Rostislav Skrabana, Mark Witmer, Joshua Ballard, Loic Martin, Petr Skladal, George Korza, Ite Laird-Offringa, Charlene S. Lee, Abdelkrim Khadir, Frank Podlaski, Phillippe Neuner, Julie Rothacker, Ashique Rafique, Nico Dankbar, Peter Kainz, Erk Gedig, Momchilo Vuyisich, Christina Boozer, Nguyen Ly, Mark Toews, Aykut Uren, Oleksandr Kalyuzhniy, Kenneth Lewis, Eugene Chomey, Brian J. Pak, David G. Myszka, A global benchmark study using affinity-based biosensors, Analytical Biochemistry, Volume 386, Issue 2, 15 March 2009, Pages 194-216, ISSN 0003-2697, DOI: 10.1016/j.ab.2008.11.021. (http://www.sciencedirect.com/science/article/B6W9V-4V17CSP-2/2/96868376b6679005f6f07057dda79e8a)|
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|Deposited By:||Tony Diaz|
|Deposited On:||14 Sep 2009 23:22|
|Last Modified:||26 Dec 2012 11:18|
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