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Nonenzymatic sequence-specific methyl transfer to single-stranded DNA

Iverson, Brent L. and Dervan, Peter B. (1988) Nonenzymatic sequence-specific methyl transfer to single-stranded DNA. Proceedings of the National Academy of Sciences of the United States of America, 85 (13). pp. 4615-4619. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:IVEpnas98

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Abstract

2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template DNA predominantly at a single guanine position. The mechanism involves methyl-group transfer from sulfur on the modified deoxyuridine of the extended primer to N-7 of guanine on the template DNA. This raises the possibility for the design and synthesis of a nonenzymatic class of sequence-specific methyl-transferases for DNA.


Item Type:Article
Additional Information:Copyright © 1988 by the National Academy of Sciences. Contributed by Peter B. Dervan, February 24, 1988. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:IVEpnas98
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:IVEpnas98
Alternative URL:http://www.pnas.org/cgi/content/abstract/85/13/4615
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1557
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Jan 2006
Last Modified:14 Nov 2014 19:19

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