Cantacessi, C. and Zou, F. C. and Hall, R. S. and Zhong, W. and Jex, A. R. and Campbell, B. E. and Ranganathan, S. and Sternberg, P. W. and Zhu, X. Q. and Gasser, R. B. (2009) Bioinformatic analysis of abundant, gender-enriched transcripts of adult Ascaris suum (Nematoda) using a semi-automated workflow platform. Molecular and Cellular Probes, 23 (5). pp. 205-217. ISSN 0890-8508 http://resolver.caltech.edu/CaltechAUTHORS:20090903-140152794
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Expressed sequence tag (EST) data representing transcripts with a high level of differential hybridization in suppressive-subtractive hybridization (SSH)-based microarray analysis between adult female and male Ascaris suum were subjected to detailed bioinformatic analysis. A total of 361 ESTs clustered into 209 sequences, of which 52 and 157 represented transcripts that were enriched in female and male A. suum, respectively. Thirty (57.7%) of the ‘female’ subset of 52 sequences had orthologues/homologues in other parasitic nematodes and/or Caenorhabditis elegans, 13 (25%) exclusively in other parasitic nematodes and nine (17.3%) had no match in any other organism for which sequence data are currently available; the C. elegans orthologues encoded molecules involved in reproduction as well as embryonic and gamete development, such as vitellogenins and chitin-binding proteins. Of the ‘male’ subset of 157 sequences, 73 (46.5%) had orthologues/homologues in other parasitic nematodes and/or C. elegans, 57 (37.5%) in other parasitic nematodes only, and 22 (14.5%) had no significant similarity match in any other organism; the C. elegans orthologues encoded predominantly major sperm proteins (MSPs), kinases and phosphatases, actins, myosins and an Ancylostoma secreted protein-like molecule. The findings of the present study should support further genomic investigations of A. suum.
|Additional Information:||© 2009 Elsevier B.V. Received 27 January 2009; accepted 16 March 2009. Available online 8 April 2009. This workwas supported by the Australian Research Council, the Australian Academy of Science and the Australian–American Fulbright Commission (RBG), the Howard Hughes Medical Institute and WormBase (PWS), the China National Science Funds for Distinguished Young Scientists (grant no. 30225033), the National Natural Science Foundation of China (grant no. 30671578), and the Natural Science Foundation of Guangdong Province (key project, grant no. 36835) (XQZ). CC is the grateful recipient of International Postgraduate Research Scholarship (IPRS) from the Australian Government and a fee-remission scholarship through the University of Melbourne, and the Sir Ian Clunies Ross Prize (2008) and Dr Sue Newton (2009) Award from the Faculty of Veterinary Science, The University of Melbourne. Note: New nucleotide sequence data used in this paper have been deposited in the GenBank database under accession numbers GO254595–GO254953.|
|Subject Keywords:||Ascaris suum; Gender-enriched; Suppressive-subtractive hybridization; Caenorhabditis elegans; Bioinformatics; Gene ontology; Genetic interactions|
|Official Citation:||C. Cantacessi, F.C. Zou, R.S. Hall, W. Zhong, A.R. Jex, B.E. Campbell, S. Ranganathan, P.W. Sternberg, X.Q. Zhu, R.B. Gasser, Bioinformatic analysis of abundant, gender-enriched transcripts of adult Ascaris suum (Nematoda) using a semi-automated workflow platform, Molecular and Cellular Probes, Volume 23, Issue 5, October 2009, Pages 205-217, ISSN 0890-8508, DOI: 10.1016/j.mcp.2009.03.003. (http://www.sciencedirect.com/science/article/B6WNC-4W1BV61-1/2/63296bb4d6cfdd90f5510e5184edfc7e)|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||22 Sep 2009 18:15|
|Last Modified:||26 Dec 2012 11:19|
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