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Design of artificial sequence-specific DNA bending ligands

Liberles, David A. and Dervan, Peter B. (1996) Design of artificial sequence-specific DNA bending ligands. Proceedings of the National Academy of Sciences of the United States of America, 93 (18). pp. 9510-9514. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LIBpnas96

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Abstract

Proteins that bend DNA are important regulators of biological processes. Sequence-specific DNA bending ligands have been designed that bind two noncontiguous sites in the major groove and induce a bend in the DNA. An oligonucleotide containing pyrimidine segments separated by a central variable linker domain simultaneously binds by triple helix formation two 15-bp purine tracts separated by 10 hp. Bend angles of 61 degrees, 50 degrees, and 38 degrees directed towards the minor groove were quantitated by phasing analysis for linkers of four, five, and six T residues, respectively. The design and synthesis of nonnatural architectural factors may provide a new class of reagents for use in biology and human medicine.


Item Type:Article
Additional Information:Copyright © 1996 by the National Academy of Sciences. Contributed by Peter B. Dervan, April 15, 1996. We are grateful for support from the National Institutes of Health (Grant GM 51747). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:TRIPLE-HELIX FORMATION; EQUILIBRIUM ASSOCIATION CONSTANTS; SITE-SPECIFIC RECOMBINATION; GEL-ELECTROPHORESIS; CRYSTAL-STRUCTURE; GENE-EXPRESSION; STRANDED-DNA; PROTEIN; TRANSCRIPTION; LENGTH
Record Number:CaltechAUTHORS:LIBpnas96
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LIBpnas96
Alternative URL:http://www.pnas.org/cgi/content/abstract/93/18/9510
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1571
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Jan 2006
Last Modified:14 Nov 2014 19:19

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