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Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear

Bok, Jinwoong and Wu, Doris K. and Bronner, Marianne E. (2005) Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development, 132 (9). pp. 2115-2124. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20090917-092638969

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Abstract

An early and crucial event in vertebrate inner ear development is the acquisition of axial identities that in turn dictate the positions of all subsequent inner ear components. Here, we focus on the role of the hindbrain in establishment of inner ear axes and show that axial specification occurs well after otic placode formation in chicken. Anteroposterior (AP) rotation of the hindbrain prior to specification of this axis does not affect the normal AP orientation and morphogenesis of the inner ear. By contrast, reversing the dorsoventral (DV) axis of the hindbrain results in changing the DV axial identity of the inner ear. Expression patterns of several ventrally expressed otic genes such as NeuroD, Lunatic fringe (Lfng) and Six1 are shifted dorsally, whereas the expression pattern of a normally dorsal-specific gene, Gbx2, is abolished. Removing the source of Sonic Hedgehog (SHH) by ablating the floor plate and/or notochord, or inhibiting SHH function using an antibody that blocks SHH bioactivity results in loss of ventral inner ear structures. Our results indicate that SHH, together with other signals from the hindbrain, are important for patterning the ventral axis of the inner ear. Taken together, our studies suggest that tissue(s) other than the hindbrain confer AP axial information whereas signals from the hindbrain are necessary and sufficient for the DV axial patterning of the inner ear.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.01796DOIUNSPECIFIED
http://dev.biologists.org/cgi/content/abstract/132/9/2115PublisherUNSPECIFIED
Additional Information:© The Company of Biologists Ltd 2005. Accepted 18 February 2005. First published online 23 March 2005. We thank Drs Susan Sullivan and Thomas Friedman for critical reading of the manuscript. M.B.F. is supported by NIH RO1 DE016459. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/132/9/2115/DC1
Funders:
Funding AgencyGrant Number
NIHRO1 DE016459
Subject Keywords:Inner ear; Axis; Axial specification; Induction; Hindbrain; Rhombomere; Sonic hedgehog; SHH; Chicken
Record Number:CaltechAUTHORS:20090917-092638969
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20090917-092638969
Official Citation:Jinwoong Bok, Marianne Bronner-Fraser, and Doris K. Wu Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear First published online on 1 May 2005 Development 132, 10.1242/dev.01796 (2005)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15901
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Sep 2009 18:44
Last Modified:26 Dec 2012 11:23

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