CaltechAUTHORS
  A Caltech Library Service

The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo

Baugh, L. Ryan and Hill, Andrew A. and Claggett, Julia M. and Hill-Harfe, Kate and Wen, Joanne C. and Slonim, Donna K. and Brown, Eugene L. and Hunter, Craig P. (2005) The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo. Development, 132 (8). pp. 1843-1854. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20090917-100109169

[img]
Preview
PDF - Published Version
See Usage Policy.

572Kb
[img] Other (Supplemental data archive (.sit): Self Extracting Archive, with readme file) - Supplemental Material
See Usage Policy.

2159Kb
[img]
Preview
Image (JPEG) (Fig. S1. QT cluster analysis of microarray data for three genotypes across 10 time points) - Supplemental Material
See Usage Policy.

1224Kb
[img]
Preview
Image (JPEG) (Fig. S2. Wild-type and mutant temporal expression patterns for validated target genes) - Supplemental Material
See Usage Policy.

636Kb
[img] Video (QuickTime) (Movie 1. Three-dimensional projection of pal-1) - Supplemental Material
See Usage Policy.

633Kb
[img] Video (QuickTime) (Movie 2. Three-dimensional projection of vab-7) - Supplemental Material
See Usage Policy.

621Kb
[img] Video (QuickTime) (Movie 3. Three-dimensional projection of cwn-1) - Supplemental Material
See Usage Policy.

1119Kb
[img] Video (QuickTime) (Movie 4. Three-dimensional projection of C55C2) - Supplemental Material
See Usage Policy.

1085Kb
[img] Video (QuickTime) (Movie 5. Three-dimensional projection of unc-120) - Supplemental Material
See Usage Policy.

972Kb
[img] Video (QuickTime) (Movie 6. Three-dimensional projection of hnd-1) - Supplemental Material
See Usage Policy.

1328Kb
[img] Video (QuickTime) (Movie 7. Three-dimensional projection of hlh-1) - Supplemental Material
See Usage Policy.

770Kb
[img] Video (QuickTime) (Movie 8. Three-dimensional projection of elt-3) - Supplemental Material
See Usage Policy.

974Kb
[img] Video (QuickTime) (Movie 9. Three-dimensional projection of mab-21) - Supplemental Material
See Usage Policy.

1066Kb
[img] Video (QuickTime) (Movie 10. Three-dimensional projection of nhr-25) - Supplemental Material
See Usage Policy.

857Kb
[img] Video (QuickTime) (Movie 11. Three-dimensional projection of nob-1) - Supplemental Material
See Usage Policy.

782Kb
[img] Video (QuickTime) (Movie 12. Three-dimensional projection of tbx-8) - Supplemental Material
See Usage Policy.

706Kb
[img] Video (QuickTime) (Movie 13. Three-dimensional projection of tbx-9) - Supplemental Material
See Usage Policy.

1245Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20090917-100109169

Abstract

Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres. pal-1-dependent C-lineage expression was verified for select candidate target genes by reporter gene analysis, though many of the target genes are expressed in additional lineages as well. The set of validated target genes includes 12 transcription factors, an uncharacterized wingless ligand and five uncharacterized genes. Phenotypic analysis demonstrates that the identified PAL-1 target genes affect specification, differentiation and morphogenesis of C-lineage cells. In particular, we show that cell fate-specific genes (or tissue identity genes) and a posterior HOX gene are activated in lineage-specific fashion. Transcription of targets is initiated in four temporal phases, which together with their spatial expression patterns leads to a model of the regulatory network specified by PAL-1.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.01782DOIUNSPECIFIED
http://dev.biologists.org/cgi/content/abstract/132/8/1843PublisherUNSPECIFIED
Additional Information:© The Company of Biologists Ltd 2005. Accepted 3 February 2005. First published online 16 March 2005. Strain JK3363 was kindly provided by Judith Kimble. We thank Shai Shen-Orr for bioinformatics support. This work was funded by an NIH GM64429 to C.P.H. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/132/8/1843/DC1
Funders:
Funding AgencyGrant Number
NIHGM64429
Subject Keywords:C. elegans; Regulatory network; Patterning; Embryonic; Cell fate specification
Record Number:CaltechAUTHORS:20090917-100109169
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20090917-100109169
Official Citation:L. Ryan Baugh, Andrew A. Hill, Julia M. Claggett, Kate Hill-Harfe, Joanne C. Wen, Donna K. Slonim, Eugene L. Brown, and Craig P. Hunter The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo First published online on 15 Apr 2005 Development 132, 10.1242/dev.01782 (2005)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15903
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Sep 2009 18:42
Last Modified:26 Dec 2012 11:23

Repository Staff Only: item control page