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Neural crest induction in Xenopus: evidence for a two-signal model

LaBonne, Carole and Bronner, Marianne E. (1998) Neural crest induction in Xenopus: evidence for a two-signal model. Development, 125 (13). pp. 2403-2414. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20090918-134232858

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Abstract

We have investigated the molecular interactions underlying neural crest formation in Xenopus. Using chordin overexpression to antagonize endogenous BMP signaling in whole embryos and explants, we demonstrate that such inhibition alone is insufficient to account for neural crest induction in vivo. We find, however, that chordin-induced neural plate tissue can be induced to adopt neural crest fates by members of the FGF and Wnt families, growth factors that have previously been shown to posteriorize induced neural tissue. Overexpression of a dominant negative XWnt-8 inhibits the expression of neural crest markers, demonstrating the necessity for a Wnt signal during neural crest induction in vivo. The requirement for Wnt signaling during neural crest induction is shown to be direct, whereas FGF-mediated neural crest induction may be mediated by Wnt signals. Overexpression of the zinc finger transcription factor Slug, one of the earliest markers of neural crest formation, is insufficient for neural crest induction. Slug-expressing ectoderm will generate neural crest in the presence of Wnt or FGF-like signals, however, bypassing the need for BMP inhibition in this process. A two-step model for neural crest induction is proposed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/125/13/2403PublisherUNSPECIFIED
Additional Information:Copyright © 1998 by Company of Biologists. Accepted 7 April; published on WWW 3 June 1998. The authors gratefully acknowledge A. Collazo, A. Knecht, C. Baker, S. Fraser and T. Moreno for critical reading of the manuscript; A. Gross and members of the laboratory for helpful comments and suggestions; and Eddy DeRobertis, Randy Moon, Rob Grainger, Gerry Thompsen, Jonathan Slack, Robert Davis, Peter Vize, Doug Melton and Roberto Mayor for providing reagents. C. L. is a fellow of the American Cancer Society. This work was also supported by USPHS NS36585 and NS34671 to M. B. F.
Funders:
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
NIHNS36585
NIHNS34671
Subject Keywords:Neural crest; Xenopus; Slug; BMP; Wnt; FGF; Chordin; Molecular interaction
Record Number:CaltechAUTHORS:20090918-134232858
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20090918-134232858
Official Citation:C LaBonne, and M Bronner-Fraser Neural crest induction in Xenopus: evidence for a two-signal model Development 125: 2403-2414.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:15982
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Sep 2009 18:31
Last Modified:26 Dec 2012 11:24

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