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Identification of discrete polyadenylate-containing RNA components transcribed from HeLa cell mitochondrial DNA

Ojala, Deanna and Attardi, Giuseppe (1974) Identification of discrete polyadenylate-containing RNA components transcribed from HeLa cell mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America, 71 (2). pp. 563-567. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:OJApnas74

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Abstract

Polyacrylamide gel electrophoresis and sedimentation analysis under denaturing conditions of poly(A)-containing RNA from the polysome region of the sedimentation pattern of a HeLa-cell mitochondrial lysate has revealed the occurrence of a discrete RNA component, which sediments in the native state with a sedimentation constant of about 7 S. From the sedimentation behavior under native and denaturing conditions and the poly(A) content, a molecular weight of about 9 x 10(4) has been estimated for this component. RNA·DNA hybridization experiments have indicated that this component is coded for by the light strand of mitochondrial DNA. Evidence for the occurrence of a poly(A)-containing RNA component sedimenting at about 9 S and coded for by the heavy strand has also been obtained.


Item Type:Article
Additional Information:Copyright © 1974 by the National Academy of Sciences. Communicated by Jerome Vinograd, October 1, 1973. The help of Mrs. A. Drew and Miss G. Engel is gratefully acknowledged. Poly(dT)-cellulose was a gift of Dr. Joseph Kates and actinomycin D, a gift of Merck, Sharp & Dohme. These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726).
Subject Keywords:actinomycin D; denaturation; poly(dT)-cellulose; polyacrylamide gel; separated strands; RNA-DNA hybridization
Record Number:CaltechAUTHORS:OJApnas74
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:OJApnas74
Alternative URL:http://www.pnas.org/cgi/content/abstract/71/2/563
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1615
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Feb 2006
Last Modified:26 Dec 2012 08:45

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