Hong, Weizhe and Zhu, Haitao and Potter, Christopher J. and Barsh, Gabrielle and Kurusu, Mitsuhiko and Zinn, Kai and Luo, Liqun (2009) Leucine-rich repeat transmembrane proteins instruct discrete dendrite targeting in an olfactory map. Nature Neuroscience, 12 (12). pp. 1542-1550. ISSN 1097-6256 http://resolver.caltech.edu/CaltechAUTHORS:20091211-105949058
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Abstract
Olfactory systems utilize discrete neural pathways to process and integrate odorant information. In Drosophila, axons of first-order olfactory receptor neurons (ORNs) and dendrites of second-order projection neurons (PNs) form class-specific synaptic connections at ~50 glomeruli. The mechanisms underlying PN dendrite targeting to distinct glomeruli in a three-dimensional discrete neural map are unclear. We found that the leucine-rich repeat (LRR) transmembrane protein Capricious (Caps) was differentially expressed in different classes of PNs. Loss-of-function and gain-of-function studies indicated that Caps instructs the segregation of Caps-positive and Caps-negative PN dendrites to discrete glomerular targets. Moreover, Caps-mediated PN dendrite targeting was independent of presynaptic ORNs and did not involve homophilic interactions. The closely related protein Tartan was partially redundant with Caps. These LRR proteins are probably part of a combinatorial cell-surface code that instructs discrete olfactory map formation.
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| Additional Information: | © 2009 Nature Publishing Group. Received 10 August; accepted 1 October; published online 15 November 2009. We thank A. Nose (University of Tokyo), S. Cohen (Temasek Life Sciences Laboratory), M. Freeman (Medical Research Council Laboratory of Molecular Biology), S. Hayashi (RIKEN Center for Developmental Biology) and M. Milan (Icrea and Parc Cientific de Barcelona) for fly stocks and reagents; the Bloomington, Szeged, Kyoto and Harvard Stock Centers for fly stocks; M. Spletter for making antennal lobe schemes; and T. Clandinin, K. Miyamichi, M. Spletter, L. Sweeney, J. Wu, X. Yu, D. Berdink and other laboratory members for comments and discussions. This work was supported by US National Institutes of Health grant R01-DC005982. L.L. is an investigator of the Howard Hughes Medical Institute. Author contributions: W.H. performed most of the experiments and analyzed the data. H.Z. initiated the overexpression screen. C.J.P. provided the GH146-Flp transgenic fly line. G.B. assisted in some experiments. M.K. and K.Z. provided the database and collection of fly strains for the overexpression screen. W.H. and L.L. designed the experiments and wrote the paper. | ||||||
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| Record Number: | CaltechAUTHORS:20091211-105949058 | ||||||
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:20091211-105949058 | ||||||
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| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 16960 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Tony Diaz | ||||||
| Deposited On: | 05 Jan 2010 19:18 | ||||||
| Last Modified: | 26 Dec 2012 11:38 |
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