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Two Different Forms of Arousal in Drosophila Are Oppositely Regulated by the Dopamine D1 Receptor Ortholog DopR via Distinct Neural Circuits

Lebestky, Tim and Chang, Jung-Sook C. and Dankert, Heiko and Zelnik, Lihi and Kim, Young-Cho and Han, Kyung-An and Wolf, Fred W. and Perona, Pietro and Anderson, David J. (2009) Two Different Forms of Arousal in Drosophila Are Oppositely Regulated by the Dopamine D1 Receptor Ortholog DopR via Distinct Neural Circuits. Neuron, 64 (4). pp. 522-536. ISSN 0896-6273. http://resolver.caltech.edu/CaltechAUTHORS:20100106-144427131

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Abstract

Arousal is fundamental to many behaviors, but whether it is unitary or whether there are different types of behavior-specific arousal has not been clear. In Drosophila, dopamine promotes sleep-wake arousal. However, there is conflicting evidence regarding its influence on environmentally stimulated arousal. Here we show that loss-of-function mutations in the D1 dopamine receptor DopR enhance repetitive startle-induced arousal while decreasing sleep-wake arousal (i.e., increasing sleep). These two types of arousal are also inversely influenced by cocaine, whose effects in each case are opposite to, and abrogated by, the DopR mutation. Selective restoration of DopR function in the central complex rescues the enhanced stimulated arousal but not the increased sleep phenotype of DopR mutants. These data provide evidence for at least two different forms of arousal, which are independently regulated by dopamine in opposite directions, via distinct neural circuits.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.neuron.2009.09.031 DOIUNSPECIFIED
http://linkinghub.elsevier.com/retrieve/pii/S0896627309007429PublisherUNSPECIFIED
http://www.cell.com/neuron/retrieve/pii/S0896627309007429PublisherUNSPECIFIED
http://www.ncbi.nlm.nih.gov/pubmed/19945394PubMed CentralUNSPECIFIED
Additional Information:© 2009 Elsevier Inc. Accepted 16 September 2009. Published: November 25, 2009. Available online 25 November 2009. We thank Ulrike Heberlein for sharing unpublished data and providing the manifold for the puff-o-mat, Martin Heisenberg and members of the Anderson lab for helpful comments on the manuscript, Eric Hoopfer for help with imaging, Michael Reiser for contributions to software development, Mary Wahl for assistance with screening, Tim Tayler for sharing Gal4 stocks prior to publication, Shilpa Jeeda for maintenance of stocks, Gaby Mosconi for lab management, and Gina Mancuso for Administrative Support. Fly stocks were obtained from the Bloomington and Harvard stock centers and also generously provided by Doug Armstrong. T.J.L. was a fellow of the Jane Coffin Childs Foundation and H.D. a fellow of the Alexander von Humboldt Association. Supported in part by an NSF FIBR grant to D.J.A., P.P., and Michael Dickinson. D.J.A. is an Investigator of the Howard Hughes Medical Institute. This paper is dedicated to the late Seymour Benzer.
Funders:
Funding AgencyGrant Number
Jane Coffin Childs Foundation UNSPECIFIED
Alexander von Humboldt Association UNSPECIFIED
NSF Frontiers in Integrative Biological Research (FIBR) UNSPECIFIED
Subject Keywords:cellbio; sysbio; molneuro
Record Number:CaltechAUTHORS:20100106-144427131
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100106-144427131
Official Citation:Two Different Forms of Arousal in Drosophila Are Oppositely Regulated by the Dopamine D1 Receptor Ortholog DopR via Distinct Neural Circuits Lebestky, Tim; Chang, Jung-Sook C.; Dankert, Heiko; Zelnik, Lihi; Kim, Young-Cho; Han, Kyung-An; Wolf, Fred W.; Perona, Pietro; Anderson, David J. doi:10.1016/j.neuron.2009.09.031 (volume 64 issue 4 pp.522 - 536)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17085
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Jan 2010 00:42
Last Modified:26 Dec 2012 11:40

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