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Polarization modulation laser scanning microscopy: A powerful tool to image molecular orientation and order

Gupta, Vinay K. and Kornfield, Julia A. (1994) Polarization modulation laser scanning microscopy: A powerful tool to image molecular orientation and order. Review of Scientific Instruments, 65 (9). pp. 2823-2828. ISSN 0034-6748. http://resolver.caltech.edu/CaltechAUTHORS:GUPrsi94

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Abstract

To image the orientational order in a broad class of biological and manufactured materials, a new microscope has been developed that integrates laser scanning microscopy with polarization modulation polarimetry. Polarimetry allows quantitative characterization of the molecular orientation and the degree of order through characterization of optical anisotropy. Combined with laser scanning microscopy, it is used here to image the anisotropy with high spatial resolution, sensitivity, and speed. The design of the microscope is presented; and the vast improvement in sensitivity achieved using PM-LSM over conventional polarization microscopy is illustrated by imaging the linear dichroism of ultrathin Langmuir–Blodgett polymer films. PM-LSM allows imaging of the magnitude and orientation of linear dichroism in films as thin as three molecular layers (~66 Å) at high resolution by rastering a diffraction limited spot of laser light across the sample. The rate of image acquisition is over 2000 pixels/s, two to three orders of magnitude faster than the previous methods of imaging optical anisotropy.


Item Type:Article
Additional Information:Copyright © 1994 American Institute of Physics. (Received 18 April 1994; accepted 17 May 1994) This work was supported by Raychem, and the Caltech Consortium in Chemistry and Chemical Engineering: E. I. duPont de Nemours, Eastman Kodak, and 3M. The authors thank Dr. A. Ferencz and Professor G. Wegner at the Max-Planck-Institut-fur-Polymerforschung for providing the LB films. We also thank J. Revel at the Division of Biology at Caltech and Dr. P. Bauerschmidt at Zeiss for their support in modifying the Zeiss LSM, and Mr. A. Kratel for his contributions to the design of the polarization-modulating unit.
Subject Keywords:MOLECULAR ORIENTATION; SCANNING LIGHT MICROSCOPY; IMAGE FORMING; SPATIAL RESOLUTION; POLARIZATION; OPTICAL ANISOTROPY; SENSITIVITY; LANGMUIR–BLODGETT FILMS; DICHROISM
Record Number:CaltechAUTHORS:GUPrsi94
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GUPrsi94
Alternative URL:http://dx.doi.org/10.1063/1.1144622
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1714
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:13 Feb 2006
Last Modified:26 Dec 2012 08:45

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