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Synthesis of Long, Representative DNA Copies of the Murine RNA Tumor Virus Genome

Rothenberg, Ellen and Baltimore, David (1976) Synthesis of Long, Representative DNA Copies of the Murine RNA Tumor Virus Genome. Journal of Virology, 17 (1). pp. 168-174. ISSN 0022-538X.

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Virions of Moloney murine leukemia virus can synthesize two classes of DNA molecules complementary to their 70S RNA. One class consists of molecules about 200 nucleotides long, which are of limited sequence complexity; these molecules are formed preferentially if the dNTP concentration during the reaction is low. The second class consists of very heterogeneous DNA molecules with weight-average size of about 1,000 nucleotides containing at least 70% of the viral RNA sequences in approximately equal concentration. The longest of these molecules can be 5,000 nucleotides long. This second class of DNA is formed in large amounts only in reactions containing dNTP concentrations of 0.2 mM or higher. In such reactions after 24 h of incubation, at least 35% of the input RNA is represented in DNA copies. The ability to make long, representative DNA transcripts of tumor virus RNA provides a source of excellent probes for molecular hybridization.

Item Type:Article
Additional Information:Copyright © 1976, American Society for Microbiology. Reprinted with permission. Received for publication 23 July 1975 This work was supported by a contract from the Virus Cancer Program of the National Cancer Institute and by Public Health Service grant CA 14051 from the National Cancer Institute. E. R. is a predoctoral fellow of the National Science Foundation. D. B. is an American Cancer Society Research Professor.
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ID Code:1731
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Deposited On:14 Feb 2006
Last Modified:26 Dec 2012 08:45

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