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C. elegans BED domain transcription factor BED-3 controls lineage-specific cell proliferation during organogenesis

Inoue, Takao and Sternberg, Paul W. (2010) C. elegans BED domain transcription factor BED-3 controls lineage-specific cell proliferation during organogenesis. Developmental Biology, 338 (2). pp. 226-236. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20100303-094838992

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Abstract

The control of cell division is critical to organogenesis, but how this control is achieved is not fully understood. We found that mutations in bed-3, encoding a BED Zn-finger domain transcription factor, confer a phenotype where a specific set of cell divisions during vulval organogenesis is lost. Unlike general cell cycle regulators in Caenorhabditis elegans, the function of bed-3 is restricted to specific lineages. Transcriptional reporters suggest that bed-3 is expressed in a limited number of cell types including vulval cells whose divisions are affected in bed-3 mutants. A bed-3 mutation also affects the expression pattern of the cdh-3 cadherin gene in the vulva. The phenotype of bed-3 mutants is similar to the phenotype caused by mutations in cog-1 (Nkx6), a component of a gene regulatory network controlling cell type specific gene expression in the vulval lineage. These results suggest that bed-3 is a key component linking the gene regulatory network controlling cell-type specification to control of cell division during vulval organogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2009.12.005DOIUNSPECIFIED
Additional Information:© 2009 Elsevier Inc. Received 22 April 2009; revised 9 November 2009; accepted 4 December 2009. Available online 17 December 2009. We thank J. Gruber, Ng L.F., B. Halliwell, Shen Y.Q. and Fu X.Y. for assistance and resources at NUS. We thank J. Fernandes and T. Ririe for comments on the manuscript, and members of the Sternberg laboratory for helpful discussions. T.I. was supported by fellowship DRG-1646 from the Damon Runyon Cancer Research Foundation. This work was supported by the HHMI, with which P.W.S. is an investigator. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR).
Funders:
Funding AgencyGrant Number
Damon Runyon Cancer Research Foundation DRG-1646
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIH National Center for Research Resources (NCRR) UNSPECIFIED
Subject Keywords:C. elegans; Organogenesis; Cell cycle regulation; Cell division; Cell proliferation; BED domain; Zinc finger; Transcription; Gene regulatory network (GRN)
Record Number:CaltechAUTHORS:20100303-094838992
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20100303-094838992
Official Citation:Takao Inoue, Paul W. Sternberg, C. elegans BED domain transcription factor BED-3 controls lineage-specific cell proliferation during organogenesis, Developmental Biology, Volume 338, Issue 2, 15 February 2010, Pages 226-236, ISSN 0012-1606, DOI: 10.1016/j.ydbio.2009.12.005. (http://www.sciencedirect.com/science/article/B6WDG-4XY9DMK-3/2/3acf73443c32e3bc8aeb0cc8ed4bf19f)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:17638
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Mar 2010 21:59
Last Modified:26 Dec 2012 11:48

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