Morris, Christina J. and Kim, Young M. and Perkins, Karin E. and Lidstrom, Mary E. (1995) Identification and nucleotide sequences of mxaA, mxaC, mxaK, mxaL, and mxaD genes from Methylobacterium extorquens AM1. Journal of Bacteriology, 127 (23). pp. 6825-6831. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:MORjbact95
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The DNA sequence for a 4.4-kb HindIII-XhoI Methylobacterium extorquens AM1 DNA fragment that is known to contain three genes (mxaAKL) involved in incorporation of calcium into methanol dehydrogenase (I. W. Richardson and C. Anthony, Biochem. J. 287:709-7115, 1992) was determined. Five complete open reading frames and two partial open reading frames were found, suggesting that this region contains previously unidentified genes. A combination of sequence analysis, mutant complementation data, and gene expression studies showed that these genes correspond to mxaSACKLDorf1. Of the three previously unidentified genes (mxaC, mxaD, and orf1), mutant complementation studies showed that mxaC is required for methanol oxidation, while the function of the other two genes is still unknown.
|Additional Information:||Copyright © 1995, American Society for Microbiology Received 3 April 1995/Accepted 14 September 1995 This work was supported by grants from the NIH (GM36296) and the DOE (DEFG03-87ER13753). We thank R. S. Hanson for supplying SM29, and we are especially grateful to G. A. Petsko for assistance in the threading analysis of the mxaACKLD gene products.|
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|Deposited On:||18 Feb 2006|
|Last Modified:||26 Dec 2012 08:46|
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