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Identification of a Second Collagen-Like Glycoprotein Produced by Bacillus anthracis and Demonstration of Associated Spore-Specific Sugars

Waller, Lashanda N. and Stump, Michael J. and Fox, Karen F. and Harley, William H. and Fox, Alvin and Stewart, George C. and Shahgholi, Mona (2005) Identification of a Second Collagen-Like Glycoprotein Produced by Bacillus anthracis and Demonstration of Associated Spore-Specific Sugars. Journal of Bacteriology, 187 (13). pp. 4592-4597. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:WALjbact05

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Abstract

Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A second collagen-like glycoprotein, BclB, was identified in B. anthracis. The protein moiety of this glycoprotein was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB.


Item Type:Article
Additional Information:Copyright © 2005, American Society for Microbiology. Received 7 February 2005/ Accepted 28 March 2005 The work was supported by NIH grant 1R21 AI05943 to G. Stewart and A. Fox. M. Stump was partially supported by the University of South Carolina Centenary Fund and Savannah River National Laboratory (SCUREF, WSCRC SC0178) and NIH R01 B0055201 (M. Matthews). We thank Bob Price and Jeff Davis for electron microscopic characterization of spores.
Record Number:CaltechAUTHORS:WALjbact05
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WALjbact05
Alternative URL:http://dx.doi.org/10.1128/JB.187.13.4592-4597.2005
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1852
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:20 Feb 2006
Last Modified:26 Dec 2012 08:46

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