Vaishampayan, Parag and Probst, Alexander and Krishnamurthi, Srinivasan and Ghosh, Sudeshna and Osman, Shariff and McDowall, Alasdair and Ruckmani, Arunachalam and Mayilraj, Shanmugam and Venkateswaran, Kasthuri (2010) Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. International Journal of Systematic and Evolutionary Microbiology, 60 (5). pp. 1031-1037. ISSN 1466-5026 http://resolver.caltech.edu/CaltechAUTHORS:20100628-100727986
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Five Gram-stain-positive, motile, aerobic strains were isolated from a clean room of the Kennedy Space Center where the Phoenix spacecraft was assembled. All strains are rod-shaped, spore-forming bacteria, whose spores were resistant to UV radiation up to 1000 J m^(–2). The spores were subterminally positioned and produced an external layer. A polyphasic taxonomic study including traditional biochemical tests, fatty acid analysis, cell-wall typing, lipid analyses, 16S rRNA gene sequencing and DNA–DNA hybridization studies was performed to characterize these novel strains. 16S rRNA gene sequencing and lipid analyses convincingly grouped these novel strains within the genus Bacillus as a cluster separate from already described species. The similarity of 16S rRNA gene sequences among the novel strains was >99 %, but the similarity was only about 97 % with their nearest neighbours Bacillus pocheonensis, Bacillus firmus and Bacillus bataviensis. DNA–DNA hybridization dissociation values were <24 % to the closest related type strains. The novel strains had a G+C content 35.6±0.5 mol% and could liquefy gelatin but did not utilize or produce acids from any of the carbon substrates tested. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0 and the cell-wall diamino acid was meso-diaminopimelic acid. Based on phylogenetic and phenotypic results, it is concluded that these strains represent a novel species of the genus Bacillus, for which the name Bacillus horneckiae sp. nov. is proposed. The type strain is 1P01SC^T (=NRRL B-59162^T =MTCC 9535^T).
|Additional Information:||© 2010 International Union of Microbiological Societies. The research described in this publication was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. This research was funded by a 2007 NRA ROSES grant. We are grateful to J. Rummel and C. Conley for useful discussions, J. A. Spry for encouragement and all of the members of the JPL Biotechnology and Planetary Protection Group for technical assistance. S. Frye and R. Casey from the Diversi Lab, Houston, TX, and M. Ott from the Johnson Space Center are acknowledged for genotyping of the isolates.|
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|Deposited By:||Jason Perez|
|Deposited On:||28 Jun 2010 21:47|
|Last Modified:||26 Dec 2012 12:10|
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