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Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus

Leadbetter, Jared R. and Greenberg, E. P. (2000) Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus. Journal of Bacteriology, 182 (24). pp. 6921-6926. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:LEAjbact00

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Abstract

Acyl-homoserine lactones (acyl-HSLs) serve as dedicated cell-to-cell signaling molecules in many species of the class Proteobacteria. We have addressed the question of whether these compounds can be degraded biologically. A motile, rod-shaped bacterium was isolated from soil based upon its ability to utilize N-(3-oxohexanoyl)-L-homoserine lactone as the sole source of energy and nitrogen. The bacterium was classified as a strain of Variovorax paradoxus. The V. paradoxus isolate was capable of growth on all of the acyl-HSLs tested. The molar growth yields correlated with the length of the acyl group. HSL, a product of acyl-HSL metabolism, was used as a nitrogen source, but not as an energy source. Cleavage and partial mineralization of the HSL ring were demonstrated by using radiolabeled substrate. This study indicates that some strains of V. paradoxus degrade and grow on acyl-HSL signals as the sole energy and nitrogen sources. This study provides clues about the metabolic pathway of acyl-HSL degradation by V. paradoxus.


Item Type:Article
Additional Information:Copyright © 2000, American Society for Microbiology. Received 19 May 2000/Accepted 27 September 2000 This research was supported by grants from the National Institutes of Health (GM59026), the National Science Foundation (MCB 9808308), and the Cystic Fibrosis Foundation. J.R.L. was supported in part by a traineeship from the National Institutes of Health (T32-AI07343) and in part by a National Science Foundation Postdoctoral Fellowship in the Biosciences Related to the Environment (DBI-9804278). We thank M. Parsek and A. Schaefer for many intellectual and technical discussions and E. Rus for performing quantitative amino acid chromatography.
Record Number:CaltechAUTHORS:LEAjbact00
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LEAjbact00
Alternative URL:http://jb.asm.org/cgi/content/abstract/182/24/6921
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ID Code:1884
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Deposited On:22 Feb 2006
Last Modified:26 Dec 2012 08:46

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