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RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions

Chien, Yueh-Hsiu and Davidson, Norman (1978) RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions. Nucleic Acids Research, 5 (5). pp. 1627-1637. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:CHInar78

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Abstract

Rates of formation of RNA:DNA hybrids have been measured as a function of temperature and compared to DNA:DNA duplex denaturation temperatures in 4 M sodium perchlorate, 4 M NaClO4-6 M urea, and 3 M rubidium trichioracetate solvents. The usual bell shaped curves of reaction rate versus temperature were observed. The optimal temperatures for the RNA:DNA association reaction are 5° to 12° greater than the Tm's for DNA:DNA denaturation in these solvents, just as in formamide. R-loops of φ80d3ilv DNA with E. coli rRNA can be formed at high efficiency in these solvents.


Item Type:Article
Additional Information:Copyright © 1978 Oxford University Press. Received 27 February 1978. We are grateful to Drs. R.L. Burke and W.R. Bauer for a generous gift of rubidium trichloracetate and to Drs. P. Chandler and J. Casey for advice. This research has been supported by contract N01 CP 43306 with the Virus Cancer Program of the National Cancer Institute. Y.H.C. has been supported in part by fellowship F32 CA 05099 from the National Cancer Institute.
Record Number:CaltechAUTHORS:CHInar78
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHInar78
Alternative URL:http://nar.oxfordjournals.org/cgi/content/abstract/5/5/1627
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1941
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Feb 2006
Last Modified:26 Dec 2012 08:46

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