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The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis

Smith, Lloyd M. and Fung, Steven and Hunkapiller, Michael W. and Hunkapiller, Tim J. and Hood, Leroy E. (1985) The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis. Nucleic Acids Research, 13 (7). pp. 2399-2412. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:SMInar85

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Abstract

A rapid and versatile method has been developed for the synthesis of oligonucleotides which contain an aliphatic amino group at their 5' terminus. This amino group reacts specifically with a variety of electrophiles, thereby allowing other chemical species to be attached to the ohgonucleotide. This chemistry has been utilized to synthesize several fluorescent derivatives of an oligonucleotide primer used in DNA sequence analysis by the dideoxy (enzymatic) method. The modified primers are highly fluorescent and retain their ability to specifically prime DNA synthesis. The use of these fluorescent primers in DNA sequence analysis will enable DNA sequence analysis to be automated.


Item Type:Article
Additional Information:Copyright © 1985 Oxford University Press. Received 12 December 1984; Revised and Accepted 19 March 1985. This work was supported by the Baxter Foundation. L.M.S. is a NIH postdoctoral trainee, supported by grant #GM07401. We are grateful to Drs. Stephen Kent, Suzanna Horvath, Robert Kaiser and Mr. Stephen Schultz for much advice and support. Excellent technical assistance was furnished by Ms. Jane Sanders.
Record Number:CaltechAUTHORS:SMInar85
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SMInar85
Alternative URL:http://dx.doi.org/10.1093/nar/13.7.2399
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1944
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Feb 2006
Last Modified:26 Dec 2012 08:46

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