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The 5' flanking region of human epsilon-globin gene

Baralle, Francisco E. and Shoulders, Carol C. and Goodbourn, Steven and Jeffreys, Alec and Proudfoot, Nicholas J. (1980) The 5' flanking region of human epsilon-globin gene. Nucleic Acids Research, 8 (19). pp. 4393-4404. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:BARnar80

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Abstract

The structural analysis of the 2.0 kb region upstream from the έ-globin gene has been carried out. A genomic DNA map around the gene was worked out in some detail to ensure that the cloned DNA was representative of the actual chromosomal arrangement. Furthermore, a new technique was developed to precisely map a reiterated DNA sequence present 1.5 kb to the 5' side of the gene. The complete nucleotide sequence of the 2.0 kb 5' flanking region was then determined and overlapped with the gene. The sequence included the reiterated DNA sequence which is homologous to the so-called AluI family of repeats. Unusual stretches of sequence 50 nucleotides long, where A + T represent about 90% of the bases, are present at both the 5' and 3' sides of the repeat.


Item Type:Article
Additional Information:Copyright © 1980 Oxford University Press. Received July 25, 1980.
Record Number:CaltechAUTHORS:BARnar80
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BARnar80
Alternative URL:http://nar.oxfordjournals.org/cgi/content/abstract/8/19/4393
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1945
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Feb 2006
Last Modified:26 Dec 2012 08:46

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