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Sequence organization of feline leukemis virus DNA in infected cells

Mullins, James I. and Casey, James W. and Nicolson, Margery O. and Davidson, Norman (1980) Sequence organization of feline leukemis virus DNA in infected cells. Nucleic Acids Research, 8 (15). pp. 3287-3305. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:MULnar80

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Abstract

A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV. Restriction fragments were ordered by single and double enzyme digests followed by Southern transfer (1) and hybridization with 32P-labeled viral cDNA probes. The restriction map was oriented with respect to the 5' and 3' ends of viral RNA by using a 3' specific hybridization probe. The major form of unintegrated viral DNA found was a 8.7 kb linear DNA molecule bearing a 450 bp direct long terminal redundancy (LTR) derived from both 5' and 3' viral RNA sequences. Minor, circular forms, 8.7 kb and 8.2 kb in length were also detected, the larger one probably containing two adjacent copies of the LTR and the smaller one containing one copy of the LTR. Integrated copies of FeLV are colinear with the unintegrated linear form and contain the KpnI and SmaI sites found in each LTR.


Item Type:Article
Additional Information:Copyright © 1980 Oxford University Press. Received June 18, 1980. We wish to thank Marie Krempin for her expert technical assistance. J.I.M. is a postdoctoral fellow of The American Cancer Society. This work was supported by grants CA25991 and CA26199 with the U.S.P.H.S. and, in its earlier stages, by contracts NO1 CP-7-1004 and NO1-CP-5-3500 with the Biological Carcinogenesis Branch of the Division of Cancer Cause and Prevention of the National Cancer Institute.
Record Number:CaltechAUTHORS:MULnar80
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MULnar80
Alternative URL:http://dx.doi.org/10.1093/nar/8.15.3287
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2024
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Mar 2006
Last Modified:26 Dec 2012 08:47

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