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Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA

Grossman, Lawrence I. and Parker, Richard C. and Watson, Robert M. and Chandler, Sarah E. W. and Teplitz, Marlyn (1977) Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA. Nucleic Acids Research, 4 (5). pp. 1267-1271. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:GROnar77

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Abstract

A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.


Item Type:Article
Additional Information:Dedicated to the memory of Professor Jerome Vinograd. Copyright © 1977 Oxford University Press. Received February 10, 1977. We are grateful to Mrs. Paula Berry, City of Hope National Medical Center, for help with the karyotype preparations. This work was supported by grants GM 21704 from NIH at Wayne State University and GM 15327 and CA 08014 from NIH at Caltech. This is contribution No. 5501 from the Division of Chemistry and Chemical Engineering.
Record Number:CaltechAUTHORS:GROnar77
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GROnar77
Alternative URL:http://dx.doi.org/10.1093/nar/4.5.1267
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2026
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Mar 2006
Last Modified:26 Dec 2012 08:47

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