Canaria, Christie A. and Lansford, Rusty (2010) Advanced optical imaging in living embryos. Cellular and Molecular Life Sciences , 67 (20). pp. 3489-3497. ISSN 1420-682X http://resolver.caltech.edu/CaltechAUTHORS:20101011-100354204
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Developmental biology investigations have evolved from static studies of embryo anatomy and into dynamic studies of the genetic and cellular mechanisms responsible for shaping the embryo anatomy. With the advancement of fluorescent protein fusions, the ability to visualize and comprehend how thousands to millions of cells interact with one another to form tissues and organs in three dimensions (xyz) over time (t) is just beginning to be realized and exploited. In this review, we explore recent advances utilizing confocal and multi-photon time-lapse microscopy to capture gene expression, cell behavior, and embryo development. From choosing the appropriate fluorophore, to labeling strategy, to experimental set-up, and data pipeline handling, this review covers the various aspects related to acquiring and analyzing multi-dimensional data sets. These innovative techniques in multi-dimensional imaging and analysis can be applied across a number of fields in time and space including protein dynamics to cell biology to morphogenesis.
|Additional Information:||© The Author(s) 2010. This article is published with open access at Springerlink.com This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. Received: 4 April 2010; Revised: 12 June 2010; Accepted: 15 June 2010; Published online: 8 July 2010. A grant from NIH CEGS (P50 HG004071) supported this review. The authors gratefully thank Mat Barnet for his contribution of sea urchin images, Willy Supatto for his pipeline figures, and Dave Huss for his discussions on ex ovo culture systems.|
|Subject Keywords:||Confocal; Two-photon; Microscopy; Time-lapse imaging; Embryogenesis|
|Official Citation:||Canaria, C. and R. Lansford (2010). "Advanced optical imaging in living embryos." Cellular and Molecular Life Sciences 67(20): 3489-3497.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||30 Nov 2010 21:42|
|Last Modified:||26 Dec 2012 12:31|
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