Shin, Young Shik and Ahmad, Habib and Shi, Qihui and Kim, Hyungjun and Pascal, Tod A. and Fan, Rong and Goddard, William A. III and Heath, James R. (2010) Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells. ChemPhysChem, 11 (14). pp. 3063-3069. ISSN 1439-4235 http://resolver.caltech.edu/CaltechAUTHORS:20101108-150340183
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The optimization of chemistries to enable the patterning of miniaturized DNA barcodes using microfluidics flow channels is described (see picture). Experiment and theory reveal that solvent mixtures in which counterions are strongly associated with the negatively charged DNA oligomers may be harnessed to produce high quality, high density DNA microarray patterns over a large area.
|Additional Information:||© 2010 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim. Received: July 1, 2010. Published online on August 16, 2010. This work was supported by National Cancer Institute Grant No. 5U54 CA119347 (J.R.H., P.I.) and by a gift from the Jean Perkins Foundation. H. K, T. A. P and W. A. G also acknowledge support from the WCU programs through NRF of Korea funded by the MEST (R31–2008–000–10055–0).|
|Subject Keywords:||microarrays; microfluidics; proteins; single-cell studies; surface chemistry|
|Official Citation:||Shin, Y. S., Ahmad, H., Shi, Q., Kim, H., Pascal, T. A., Fan, R., Goddard, W. A. and Heath, J. R. (2010), Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells. ChemPhysChem, 11: 3063–3069. doi: 10.1002/cphc.201000528|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||29 Nov 2010 18:18|
|Last Modified:||26 Dec 2012 12:36|
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