Banroques, Josette and Abelson, John N. (1989) PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle. Molecular and Cellular Biology, 9 (9). pp. 3710-3719. ISSN 0270-7306 http://resolver.caltech.edu/CaltechAUTHORS:BANmcb89
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The Saccharomyces cerevisiae prp mutants (prp2 through prp11) are known to be defective in pre-mRNA splicing at nonpermissive temperatures. We have sequenced the PRP4 gene and shown that it encodes a 52-kilodalton protein. We obtained PRP4 protein-specific antibodies and found that they inhibited in vitro pre-mRNA splicing, which confirms the essential role of PRP4 in splicing. Moreover, we found that PRP4 is required early in the spliceosome assembly pathway. Immunoprecipitation experiments with anti-PRP4 antibodies were used to demonstrate that PRP4 is a protein of the U4/U6 small nuclear ribonucleoprotein particle (snRNP). Furthermore, the U5 snRNP could be immunoprecipitated through snRNP-snRNP interactions in the large U4/U5/U6 complex.
|Additional Information:||Copyright © 1989, American Society for Microbiology Received 6 February 1989/Accepted 22 May 1989 We thank J. Beggs and R. Luhrmann for the generous gift of anti-PRP8 and anti-m3G antibodies and J. Woolford for providing the PRP4 yeast pJDB207/PRP4 plasmid. We are grateful to S.-C. Cheng for help with nondenaturing gels and to G. Komatsoulis and G. McFarland for help with computer analysis. We gratefully acknowledge K. Tanner, P. Fabrizio, G. McFarland, and J. Arenas for critical readings of the manuscript. We also thank S. Horvath for synthesizing oligonucleotides and C. Blagg for typing the manuscript. J.B. was a recipient of NATO and CNRS/NSF fellowships. This work was supported by Public Health Service grant GM 32637 from the National Institute of Health.|
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