Benbow, R. M. and Hutchison, C. A., III and Fabricant, J. D. and Sinsheimer, R. L. (1971) Genetic Map of Bacteriophage [var phi]X174. Journal of Virology, 7 (5). pp. 549-558. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:BENjvir71
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Bacteriophage [var phi]X174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses. The genetic map is circular with a total length of 24 × 10−4wt recombinants per progeny phage. The cistron order is D-E-F-G-H-A-B-C. High negative interference is seen, consistent with a small closed circular deoxyribonucleic acid molecule as a genome.
|Additional Information:||Copyright © 1971, American Society for Microbiology Received for publication 28 November 1970 This investigation was supported by Public Health Service grant GM13554 from the National Institute of General Medical Sciences. For the initial idea to study genetic recombination in [phi]X174, we are grateful to Paul Howard-Flanders. In addition, we thank Gloria Davis and Kay Burroughs for excellent technical assistance, Alma J. Shafer for performing several complementation tests, and the "[phi]X174 group" for critical comments on this manuscript before publication. We would also like to acknowledge a stimulating discussion about "hairpin" DNA configurations with R. Shekman.|
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|Deposited On:||13 Mar 2006|
|Last Modified:||26 Dec 2012 08:47|
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