Benbow, Robert M. and Mayol, Robert F. and Picchi, Joanna C. and Sinsheimer, Robert L. (1972) Direction of Translation and Size of Bacteriophage [var phi]X174 Cistrons. Journal of Virology, 10 (1). pp. 99-114. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:BENjvir72
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Translation of the bacteriophage [var phi]X174 genome follows cistron order D-E-F-G-H-A-B-C. To establish this, the position of a nonsense mutation on the genetic map was compared with the physical size (molecular weight) of the appropriate protein fragment generated in nonpermissive cells. Distances on the [var phi]X174 genetic map and distances on a physical map constructed from the molecular weights of [var phi]X174 proteins and protein fragments are proportional over most of the genome with the exception of the high recombination region within cistron A.
|Additional Information:||Copyright © 1972, American Society for Microbiology Received for publication 20 March 1972 We thank Paul Howard-Flanders for the initial idea to study the genetic recombination of bacteriophage [phi]X174. The excellent technical assistance of Gloria Davis, Alma Shafer, David Holmes, and Jill Fabricant Hiatt is acknowledged. A preliminary account of these results was given at the Bacteriophage Meetings, Cold Spring Harbor, 27-31 August 1970. This research was carried out with the assistance of Public Health Service training grant GM-86 and research grant GM13554 from the National Institute of General Medical Sciences.|
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|Deposited On:||13 Mar 2006|
|Last Modified:||26 Dec 2012 08:47|
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