Liebling, Michael and Vermot, Julien and Forouhar, Arian S. and Gharib, Morteza and Dickinson, Mary E. and Fraser, Scott E. (2006) Nonuniform temporal alignment of slice sequences for four-dimensional imaging of cyclically deforming embryonic structures. In: 2006 3rd IEEE International Symposium on Biomedical Imaging: Macro to Nano. IEEE International Symposium on Biomedical Imaging . IEEE , Piscataway, NJ, pp. 1156-1159. ISBN 978-0-7803-9576-3 http://resolver.caltech.edu/CaltechAUTHORS:20110210-150606480
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The temporal alignment of nongated slice-sequences acquired at different axial positions in the living embryonic zebrafish heart permits the reconstruction of dynamic, three-dimensional data. This approach overcomes the current acquisition- speed limitation of confocal microscopes for real-time three-dimensional imaging of fast processes. Current synchronization methods align and uniformly scale the data in time, but do not compensate for slight variations in the heart rhythm that occur within a heartbeat. Therefore, they impose constraints on the admissible data quality. Here, we derive a nonuniform registration procedure based on the minimization of the absolute value of the intensity difference between adjacent slice-sequence pairs. The method compensates for temporal intra-sample variations and allows the processing of a wider range of data to build functional, dynamic models of the beating embryonic heart. We show reconstructions from data acquired in living, fluorescent zebrafish embryos.
|Item Type:||Book Section|
|Additional Information:||© 2006 IEEE. Issue Date: 6-9 April 2006; Date of Current Version: 08 May 2006. We thank Sean Megason, Le Trinh, and Chris Waters for help with zebrafish preparation and advice on imaging techniques. M.L. would like to thank Michael Unser for helpful discussions and for sharing unpublished work on related topics.|
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|Official Citation:||Liebling, M.; Vermot, J.; Forouhar, A.S.; Gharib, M.; Dickinson, M.E.; Fraser, S.E.; , "Nonuniform temporal alignment of slice sequences for four-dimensional imaging of cyclically deforming embryonic structures," Biomedical Imaging: Nano to Macro, 2006. 3rd IEEE International Symposium on , vol., no., pp.1156-1159, 6-9 April 2006 doi: 10.1109/ISBI.2006.1625128 URL: http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=1625128&isnumber=34114|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Benjamin Perez|
|Deposited On:||11 Feb 2011 15:38|
|Last Modified:||11 Jul 2011 17:10|
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