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Heteroduplex study of the sequence relations between RD-114 and baboon viral RNAs

Hu, Sylvia and Davidson, Norman and Nicolson, Margery O. and McAllister, Robert M. (1977) Heteroduplex study of the sequence relations between RD-114 and baboon viral RNAs. Journal of Virology, 23 (2). pp. 345-352. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:HUSjvir77

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Abstract

The regions of sequence homology and nonhomology between the RNA genomes of RD-114 and baboon endogenous type C viruses have been mapped by an electron microscope heteroduplex study. Short complementary DNA (cDNA) copies (approximately 150 to 200 nucleotides in length) of RD-114 RNA were prepared by an endogenous synthesis; labels of polydeoxythymidylic acid [poly(dT)] were attached to the 3' ends of the cDNA molecules by a reaction catalyzed by deoxynucleotidyl terminal transferase. The cDNA-poly(dT) was hybridized to RD-114 RNA and to baboon viral RNA dimer (50 to 70S) units, and the position- of the poly(dT) labels were observed by electron microscopy. With RD-114, labels were distributed uniformly along the genome. With baboon virus RNA (monomer length, 9.5 kilobases [kb]), the regions of high homology with RD-114 cDNA were observed to lie in the intervals from 1.5 to 2.5 kb and from 3.7 to 5.5 kb from the 5' end. The relations of these heteroduplex maps to the known antigenic similarities and differences among the several viral proteins and to the genetic maps of the viruses are discussed.


Item Type:Article
Additional Information:Copyright © 1977 by the American Society for Microbiology. Received for publication 7 March 1977 This research was supported by Public Health Service contracts NO1 CP 43306 and PH 43-68-1030 from the Virus Cancer Program of the National Cancer Institute. We thank Welcome Bender for guidance and advice on terminal transferase reactions. We also thank Marie Krempin and Mary Peer for technical assistance. Contribution no. 5504 from the Department of Chemistry, California Institute of Technology, Pasadena, CA 91125.
Record Number:CaltechAUTHORS:HUSjvir77
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HUSjvir77
Alternative URL:http://pubmedcentral.gov/articlerender.fcgi?artid=515836
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ID Code:2215
Collection:CaltechAUTHORS
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Deposited On:15 Mar 2006
Last Modified:26 Dec 2012 08:48

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