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Inhibitor studies of dissimilative Fe(III) reduction by Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans")

Arnold, Robert G. and DiChristina, Thomas J. and Hoffmann, Michael R. (1986) Inhibitor studies of dissimilative Fe(III) reduction by Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans"). Applied and Environmental Microbiology, 52 (2). pp. 281-289. ISSN 0099-2240. http://resolver.caltech.edu/CaltechAUTHORS:ARNaem86

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Abstract

Aerobic respiration and dissimilative iron reduction were studied in pure, batch cultures of Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans"). Specific respiratory inhibitors were used to identify elements of electron transport chains involved in the reduction of molecular oxygen and Fe(III). When cells were grown at a high oxygen concentration, dissimilative iron reduction occurred via an abbreviated electron transport chain. The induction of alternative respiratory pathways resulted from growth at low oxygen tension (less than 0.01 atm [1 atm = 101.29 kPa]). Induced cells were capable of O2 utilization at moderately increased rates; dissimilative iron reduction was accelerated by a factor of 6 to 8. In cells grown at low oxygen tension, dissimilative iron reduction appeared to be uncoupled from oxidative phosphorylation. Models of induced and uninduced electron transport chains, including a mathematical treatment of chemical inhibition within the uninduced, aerobic electron transport system, are presented. In uninduced cells respiring anaerobically, electron transport was limited by ferrireductase activity. This limitation may disappear among induced cells.


Item Type:Article
Additional Information:Copyright © 1986 by the American Society for Microbiology. Received 21 February 1986/Accepted 7 May 1986 We are grateful to D. W. S. Westlake of the Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada, for generously providing the microorganism used in this study, Pseudomonas sp. strain 200. The taxonomic studies leading to species identification were undertaken and reported by D. Westlake and C. O. Obuekwe; we have changed its name to "Pseudomonas ferrireductans" (upon request of a reviewer) with some reluctance. We hope that its new name will provide the microorganism with deserved distinction without causing excessive confusion. We appreciate the support and encouragement of Ryszard Gajewski and Duane L. Barney. We thank Sandy Brooks, Elaine Granger, and Nancy Tomer of the Caltech Environmental Sciences and Engineering staff for secretarial and drafting assistance during manuscript preparation. This work was supported by U.S. Department of Energy contract no. DE-AS03-83ER13125 administered within the Division of Advanced Energy Projects, Office of the Basic Energy Sciences.
Record Number:CaltechAUTHORS:ARNaem86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ARNaem86
Alternative URL:http://aem.asm.org/cgi/content/abstract/52/2/281
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2216
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:15 Mar 2006
Last Modified:26 Dec 2012 08:48

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