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Regulation of Dissimilatory Fe(III) Reduction Activity in Shewanella putrefaciens

Arnold, Robert G. and Hoffmann, Michael R. and DiChristina, Thomas J. and Picardal, Flynn W. (1990) Regulation of Dissimilatory Fe(III) Reduction Activity in Shewanella putrefaciens. Applied and Environmental Microbiology, 56 (9). pp. 2811-2817. ISSN 0099-2240. http://resolver.caltech.edu/CaltechAUTHORS:ARNaem90

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Abstract

Under anaerobic conditions, Shewanella putrefaciens is capable of respiratory-chain-linked, high-rate dissimilatory iron reduction via both a constitutive and inducible Fe(III)-reducing system. In the presence of low levels of dissolved oxygen, however, iron reduction by this microorganism is extremely slow. Fe(II)-trapping experiments in which Fe(III) and O2 were presented simultaneously to batch cultures of S. putrefaciens indicated that autoxidation of Fe(II) was not responsible for the absence of Fe(III) reduction. Inhibition of cytochrome oxidase with CN– resulted in a high rate of Fe(III) reduction in the presence of dissolved O2, which suggested that respiratory control mechanisms did not involve inhibition of Fe(III) reductase activities or Fe(III) transport by molecular oxygen. Decreasing the intracellular ATP concentrations by using an uncoupler, 2,4-dinitrophenol, did not increase Fe(III) reduction, indicating that the reduction rate was not controlled by the energy status of the cell. Control of electron transport at branch points could account for the observed pattern of respiration in the presence of the competing electron acceptors Fe(III) and O2.


Item Type:Article
Additional Information:Copyright © 1990, American Society for Microbiology. Received 14 February 1990/Accepted 12 May 1990 We thank Barbara Graham and Liz Lavis for assistance in preparing the manuscript and Allison Habel for preparation of graphics.
Record Number:CaltechAUTHORS:ARNaem90
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ARNaem90
Alternative URL:http://aem.asm.org/cgi/content/abstract/56/9/2811
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2217
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:15 Mar 2006
Last Modified:26 Dec 2012 08:48

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