Van Alstyne, D. and Grant, G. F. and Simon, M. (1969) Synthesis of Bacterial Flagella: Chromosomal Synchrony and Flagella Synthesis. Journal of Bacteriology, 100 (1). pp. 283-287. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:ALSjbact69
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Synchronous cultures of Bacillus subtilis 168 M were obtained from light-density spores germinated at 46 C and grown at 37 C. This procedure synchronizes both cell division and chromosome replication. The chromosome synchrony was demonstrated by using transformation to measure changes in marker frequency during the cell cycle. The synthesis of two enzymes and of bacterial flagellar protein was also followed. All of the proteins were found to be synthesized continuously with an abrupt doubling in the rate of synthesis at a specific time in the cell cycle. The time at which the doubling occurred for each enzyme corresponded to the time at which the structural gene for the enzyme was replicated. The doubling of the rate of flagella synthesis corresponded to the time of replication of the hisA1 gene. We conclude that the genetic locus for the factors involved in the rate-limiting steps in flagella synthesis are located on the genetic map near the hisA1 locus.
|Additional Information:||Copyright © 1969 American Society for Microbiology. Received for publication 14 July 1969 We express our gratitude to J. Spizizen for valuable suggestions and for generously permitting one of us (D. V.) to make use of his laboratory facilities. This work was supported by grant no. GB-6980 from the National Science Foundation.|
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|Deposited On:||15 Mar 2006|
|Last Modified:||26 Dec 2012 08:48|
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