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Division Mutants of Bacillus subtilis: Isolation and PBS1 Transduction of Division-Specific Markers

Van Alstyne, D. and Simon, M. I. (1971) Division Mutants of Bacillus subtilis: Isolation and PBS1 Transduction of Division-Specific Markers. Journal of Bacteriology, 108 (3). pp. 1366-1379. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:ALSjbact71

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Abstract

A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four mutants in the divA group synthesize septa; however, they do so with a high frequency of error resulting in minicell production. Mapping data were obtained by scoring cotransduction frequencies using PBS1-transducing lysates. Thus, some of the genes apparently involved in septum synthesis and their order on the genome have been established. The results of a study by electron microscope of some of the mutants is also presented.


Item Type:Article
Additional Information:Copyright © 1971 American Society for Microbiology. Received for publication 13 September 1971 From a dissertation submitted by D. Van Alstyne in May 1971 to the University of California, San Diego, in partial fulfillment of the requirements for the Ph.D. degree.
Record Number:CaltechAUTHORS:ALSjbact71
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ALSjbact71
Alternative URL:http://jb.asm.org/cgi/content/abstract/108/3/1366
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2219
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:15 Mar 2006
Last Modified:26 Dec 2012 08:48

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