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Caenorhabditis elegans EVL-14/PDS-5 and SCC-3 Are Essential for Sister Chromatid Cohesion in Meiosis and Mitosis

Wang, Fang and Yoder, John and Antoshechkin, Igor and Han, Min (2003) Caenorhabditis elegans EVL-14/PDS-5 and SCC-3 Are Essential for Sister Chromatid Cohesion in Meiosis and Mitosis. Molecular and Cellular Biology, 23 (21). pp. 7698-7707. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:WANmcb03

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Abstract

Sister chromatid cohesion is fundamental for the faithful transmission of chromosomes during both meiosis and mitosis. Proteins involved in this process are highly conserved from yeasts to humans. In screenings for sterile animals with abnormal vulval morphology, mutations in the Caenorhabditis elegans evl-14 and scc-3 genes were isolated. Defects in cell divisions were observed in germ line as well as in vulval and somatic gonad lineages. Through positional cloning of these genes, we have shown that EVL-14 and SCC-3 are likely the only C. elegans homologs of the yeast sister chromatid cohesion proteins Pds5 and Scc3, respectively. Both evl-14 and scc-3 mutants displayed defects in the meiotic germ line. In evl-14 mutants, synaptonemal complexes (SCs) were detectable but more than the usual six DAPI (4',6'-diamidino-2-phenylindole)-positive structures were seen at diakinesis, suggesting that EVL-14/PDS-5 is important for the maintenance of sister chromatid cohesion in late prophase. In scc-3 mutant animals, normal SCs were not visible and ~24 DAPI-positive structures were seen at diakinesis, indicating that SCC-3 is necessary for sister chromatid cohesion. Immunostaining revealed that localization of REC-8, a homolog of the yeast meiotic cohesin subunit Rec8, to the chromosomes depends on the presence of SCC-3 but not that of EVL-14/PDS-5. scc-3 RNA interference (RNAi)-treated embryos were 100% lethal and displayed defects in cell divisions. evl-14 RNAi caused a range of phenotypes. These results indicate that EVL-14/PDS-5 and SCC-3 have functions in both mitosis and meiosis.


Item Type:Article
Additional Information:Copyright © 2003, American Society for Microbiology. Received 17 April 2003/ Returned for modification 20 May 2003/ Accepted 25 July 2003 We thank Matt Siebert for RT-PCR work on evl-14, J. Loidl for anti-REC-8 antiserum, Iva Greenwald for evl-14 alleles, Yuji Kohara for cDNA clones, Bill Wood, Jinwei Zhao, and Qian Hu for the use of equipment, and Yo Suzuki, Dan Starr, Lois Edgar, and Quinn Crawford for comments on the manuscript. Some of the strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the National Center for Research Resources of the NIH. This study was supported by grants from Shanghai Municipal Government (Division of Sci. and Tech), China's NNSF (Oversea Young Scholar Collaborative Research Award), and the NIH of the United States (RO1, GM37869). J.Y. was supported by an NIH predoctoral training grant. I.A. was a research associate and M.H. is an associate investigator of HHMI.
Record Number:CaltechAUTHORS:WANmcb03
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WANmcb03
Alternative URL:http://dx.doi.org/10.1128/MCB.23.21.7698-7707.2003
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2224
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 Mar 2006
Last Modified:26 Dec 2012 08:48

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