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13C nuclear magnetic resonance study of trehalose mobilization in yeast spores

Barton, J. K. and den Hollander, J. A. and Hopfield, J. J. and Shulman, R. G. (1982) 13C nuclear magnetic resonance study of trehalose mobilization in yeast spores. Journal of Bacteriology, 151 (1). pp. 177-185. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:BARjbact82

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Abstract

Using high-resolution 13C nuclear magnetic resonance, we examined the mobilization of endogenous trehalose in suspensions of yeast asci. Sporulation of yeast cells in [1-13C]acetate resulted in incorporation of label into the C-3 and C-4 positions of trehalose within the asci. During germination of these asci with [1-13C]glucose, the consumption of both endogenous trehalose and exogenous glucose were followed simultaneously by 13C nuclear magnetic resonance, as was the formation of glycerol and ethanol, their glycolytic and products. Time courses for carbohydrate consumption indicated that trehalose, although it decreased to 25% of its initial value upon germination, was not preferentially catabolized and did not provide the primary energy supply for germination with glucose. The ratio of trehalose to glucose catabolized was 0.09. Exogenous glucose levels appeared to regulate trehalose mobilization since trehalose was only consumed when sufficiently high levels (more than 2 mM) of glucose were present. Upon glucose depletion newly synthesized [1-13C]trehalose was observed. Nuclear magnetic resonance spectra of extracts confirmed the trehalose peak assignments and showed products of [1-13C]glucose catabolism. In addition by quantitating trehalose consumption and 2-deoxyglucose incorporation in dormant yeast asci, we found that 3.8 +/- 0.l4 molecules of 2-deoxyglucose were incorporated for each trehalose molecule consumed. Trehalose can therefore function as a carbohydrate source for ATP formation during dormancy.


Item Type:Article
Additional Information:Copyright © 1982 by the American Society for Microbiology. Received 28 July 1981/Accepted 16 February 1982 This work has been supported by Public Health Service grant AM 27121 from the National Institutes of Health and by Public Health Service research service award GM 07416 to J.K.B. from the National Institutes of Health.
Record Number:CaltechAUTHORS:BARjbact82
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BARjbact82
Alternative URL:http://jb.asm.org/cgi/content/abstract/151/1/177
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2241
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Deposited On:17 Mar 2006
Last Modified:26 Dec 2012 08:48

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